Technical Library

Recent Entries in Technical Library

• How many cells do the cell pellets contain?

  PromoCell cell pellets are made from > 1 million cells and are dissolved in 200 µl RNAlater.

• What medium should I use to grow or differentiate PromoCell Human Stem and Blood Cells?

  The recommended media for a particular cell type are specified on the respective product page (“Recommended Products”) and can be found in the Manual belonging to the cells.

• What media should I use to grow PromoCell Normal Human Cells?

  The recommended Growth Media are specified in the Manual belonging to the cells and can be also found on the product page of the cells (“Recommender products”).

• Can I aliquot the SupplementMix?

  Yes, you can aliquot the SupplementMix upon delivery and freeze down 2 or 4 individual aliquots at -20°C. This way, you can prepare smaller volumes (2 x 250 ml or 4 x 125 ml) of complete culture medium and thus extend the time you can use the medium.

• Can I trypsinize the PromoCell Normal Human Cells at 37°C?

  PromoCell recommend to trypsinize all Normal Human Cells at room temperature and to monitor the detachment under the microscope. Prolonged trypsinization at 37°C can lead to irreversible damage of the cells.

  Related Links and Documents

  • Subcultivation of primary cells
• What is the concentration of ECGS contained in Endothelial Cell Growth Media/Preadipocyte Growth Media?

  At manufacture, ECGS is adjusted to a protein content of 3 mg/ml. For Human Endothelial Cells and Microvascular Endothelial Cells the optimal concentration of ECGS is 2 ml/500 ml medium, corresponding to 6 mg extracted protein/500 ml medium. ECGS/H is additionally supplemented with 22.5 mg/ml heparin, corresponding to a final concentration of 45 mg heparin/500 ml medium.

  Related Links and Documents

  • ECGS
• Should I store the cryopreserved cells in the liquid phase or gas phase of liquid nitrogen?

  In principle, both types of liquid nitrogen storage are acceptable, each having its advantages and disadvantages.

  • Liquid phase storage provides a consistent temperature of -196°C, a longer holding time and a greater vial capacity but involves the risk of contamination issues.
  • Storage in the gas phase is very safe with respect to contaminations but the holding time of the cells is shorter and the vial capacity is reduced.
• Will RNAlater solution denature the proteins in the cell pellets?

  Yes, RNAlater Solution will denature proteins. Therefore, protein obtained from PromoCell cell pellets will be suitable for applications such as Western blotting or 2D gel electrophoresis, but not for applications that require native protein.

  Related Links and Documents

  • Cell pellets
• What is the exact localization of PromoCell’s HNEpC (C-12620)?

  Our Nasal Epithelial Cells are isolated from nasal septum or adenoids.

  Related Links and Documents

  • HNEpC
• What is the precise localization for a) HNEpC, b) HTEpC, c) HBEpC, and d) HSAEpC?

  a) HNEpC (Human Nasal Epithelial Cells) are isolated from nasal mucosa
  b) HTEpC (Human Tracheal Epithelial Cells) from the surface epithelium of trachea
  c) HBEpC from the surface epithelium of bronchie, and
  d) HSAEpC (Human Small Airway Epithelial Cells) from the distal portion of the respiratory tract in the 1 mm bronchiole area
  (comprising the cells from bronchioli and alveoli).

  Related Links and Documents

  • Human Small Airway Epithelial Cells