Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Generally, VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple effects on cell metabolism, it may also interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.
Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.
To view a list of references where this medium was used with other species/cell types please click here.
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
|Media supplement||Final supplement concentration|
|Fetal Calf Serum||0.02 ml / ml|
|Epidermal Growth Factor (recombinant human)||5 ng / ml|
|Basic Fibroblast Growth Factor (recombinant human)||10 ng / ml|
|Insulin-like Growth Factor (Long R3 IGF)||20 ng / ml|
|Vascular Endothelial Growth Factor 165 (recombinant human)||0.5 ng / ml|
|Ascorbic Acid||1 µg / ml|
|Heparin||22.5 μg / ml|
|Hydrocortisone||0.2 µg / ml|
Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.