3D Tumorsphere Medium XF

Serum-free and xeno-free medium for the isolation and 3D long-term cultivation of cancer cells.

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3D Tumorsphere Medium XF (Ready-to-use) 250 ml

3D Tumorsphere Medium XF (Ready-to-use), phenol red-free 250 ml

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Key benefits:

  • Establish 3D tumorsphere cultures directly from 2D cancer cell lines
  • Suitable for long-term routine culture of tumorspheres
  • Compatible with most commonly used cancer cell lines
  • Xeno-free and serum-free formulation

Components:

  • 3D Tumorsphere Basal Medium
  • 3D Tumorsphere SupplementMix

3D cancer spheroids and organoids are popular in vitro models that often better reflect the in vivo tumor environment than some 2D models. Within tumors and tumor spheroids, cancer stem cells (CSCs) are a small subpopulation of cells that play a key role in tumor initiation, recurrence, metastasis, and even resistance to cancer therapies. CSCs exhibit stem cell-like characteristics including self-renewal capabilities and tumorigenicity.

As a part of our Cancer Media Toolbox, our 3D Tumorsphere Medium XF is ideal for isolation and 3D long-term cultivation of CSCs. 3D Tumorsphere Medium XF supports enrichment and maintenance of cancer stem cells as well as further differentiated cancer cells with high cell proliferation rates.

The medium helps you create a reducible in vivo-like system by promoting the formation of homogenous tumorspheres through serial passaging of the 3D culture.

When it comes to the characterization of cultured CSCs, the tumorsphere formation efficiency (TFE) assay represents the gold standard. The TFE assay describes the percentage of cells within a cancer cell culture that can form a sphere from a single cell. Since only stem-like cells can form spheroids from a single cell, TFE values are a quantitative measurement that correlates with cancer metastasis and aggressiveness. In experiment where MCF-7 cells have been cultured in 3D Tumorsphere Medium XF, the TFE value significantly improved from 2% to 28% over 9 passages.

3D Tumorsphere Medium XF In Cancer Media Tool Box

The 3D Tumorsphere Medium XF is flexible enough to support different cell types tested for serial passage:

Tissue Tested cell lines Cell lines origin
Brain U-87 MG Grade IV glioblastoma / astrocytoma of the human brain
Breast MCF-7 Pleural effusion of metastatic human breast adenocarcinoma
Breast MDA-MB-231 Pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
Colon HT-29 Human colon adenocarcinoma
Connective tissue HT1080 human fibrosarcoma
Liver HepG2 Hepatocellular carcinoma of the human liver
Lung A-549 Human lung carcinoma
Pancreas Panc-1 Epithelioid carcinoma of the human pancreatic duct
Prostate LNCaP Lymph node metastasis of human prostate adenocarcinoma
Skin A-431 Epidermoid carcinoma of the human skin
3D Tumorspheres
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
3D Tumorspheres
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
3D Tumorsphere Medium - tested cell lines
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
3D Tumorsphere Medium - tested cell lines
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
3D Tumorsphere Medium - TFE
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.
3D Tumorsphere Medium - TFE
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.

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