Technical Library

Recent Entries in Technical Library

• Has PromoCell done any tests with MSC Adipogenic Differentiation Medium 2 without fibronectin? Is fibronectin coating really necessary?

  The MSCs attach even without fibronectin coating, but the risk of detachment during differentiation is much higher. We therefore strongly recommend using fibronectin-coated TC vessels.

  Related Links and Documents

  • Human Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Adipogenic Differentiation Medium 2
• I would like to know if the Lymphocyte Separation Medium (C-44010) is endotoxin tested.

  Yes, our Lymphocyte Separation Medium 1077 is endotoxin-tested. The specification for the product is < 10EU/ml.

  Related Links and Documents

  • Lymphocyte Separation Medium
• Is the PromoCell Keratinocyte Growth Medium 2 suitable for growth of primary mouse keratinocytes?

  Yes, primary mouse keratinocytes grow well in this media when you reduce the CaCl₂ concentration to 0.025 mM – 0.05 mM CaCl₂ (optimal Ca concentration for primary human keratinocytes is 0.06 mM).

  Related Links and Documents

  • Keratinocyte Growth Medium 2
• When do I need PromoCell’s Monocyte Attachment Medium (C-28051)?

  The Monocyte Attachment Medium allows for the efficient adherence selection (within 1 hr) of monocytes from freshly isolated mononuclear cells while maintaining optimal cell health. The Time-consuming and costly immunomagnetic purification of monocytes prior to DC generation is not necessary and can be skipped when using this medium.
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  Related Links and Documents

  • Instruction Manual: DC Media
  • Application Note: Generation of moDCs
  • Use of DC Attachment Medium
• How long does it take to detach primary cells with accutase (C-41310)?

  Most primary cells detach within 5-10 min at 37°C. Inactivation isn’t required but we recommend to centrifuge the cell suspension to remove accutase and EDTA before replating the cells.

  Related Links and Documents

  • Accutase Solution
• Is it possible to transfect PromoCell Normal Human Cells?

  Yes, it is possible to transfect our normal human cells. In general, primary and normal cells are much harder to transfect than immortalized cell lines. Apart from the cell type, successful transfection also depends on the culture’s age and density at transfection, the vector used, the purity of the nucleic acids, the composition of the transfection medium, and the experimental conditions.

• What’s the stability of HPC Expansion Medium XF (C-28021) after addition of Cytokine Mix E (C-39890)?

  The medium supplemented with Cytokine Mix E is stable for 2 weeks if stored protected from light at 2-8°C.

  Related Links and Documents

  • Hematopoietic Progenitor Expansion Medium XF
• Is it necessary to heat inactivate FCS for use with Normal Human Cells?

  The practice of heat inactivation was originally developed when only serum from adult animals was available. Adult serum contains high serum complement which may destroy cells under certain conditions. Heating serum (30 min, 56°C) is intended to inactivate the complement. Today, serum is often heat-inactivated without any evidence of beneficial effect. When using FCS (fetal calf serum), heat inactivation is not necessary for most cell lines or cell types. PromoCell does not use heat-inactivated FCS for the preparation of their growth media.

• Can I use my own culture medium or other commercially available media for culturing PromoCell Normal Human Cells?

  Our Normal Human Cells have been cultured and tested in our growth media and have adapted to these conditions. Using other media may yield unsatisfactory results due to suboptimal supplies of nutrients and growth factors. PromoCell can only guarantee good cell growth (as stated in the Certificate of Analysis), when the cells are grown in the recommended media.

• Does the differentiation capacity of Human Mesenchymal Stem Cells change over time? When should I best induce differentiation?

  We have tested the differentiation capacity of our hMSC into adipocytes, chondrocytes and osteoblasts over time and still see good differentiation rates after 10 population doublings, i.e., at passage 5. However, the differentiation potential declines with ongoing population doublings. To obtain optimal differentiation rates, experiments should be performed as early in culture as possible.

  Related Links and Documents

  • Mesenchymal Stem Cell Culture