Technical Library

Recent Entries in Technical Library

• Why is the growth rate of PromoCell’s HDMEC significantly reduced when I remove hydrocortisone from the Endothelial Cell Growth Medium MV Kit (C-22120)?

  Epidermal Growth Factor (EGF) and hydrocortisone have been reported to have a synergistic effect on the growth of microvascular endothelial cells. Accordingly, if you remove the hydrocortisone, the proliferation of HDMEC is clearly affected.

  Related Links and Documents

  • Endothelial Cell Growth Medium MV
• Where does PromoCell source the heart tissue for HCM isolation?

  The tissue comes from patients who underwent heart transplantation. We obtain a part of the explanted (not the transplanted) heart to prepare cardiac myocytes.

  Related Links and Documents

  • Human Cardiac Myocytes
• For how long can the cardiac myocytes (C-12810) be grown in culture?

  PromoCell guarantees 15 population doublings (PDs) if the HCM are grown in Myocyte Growth Medium. Depending on the cell lot and the culture conditions, the cells can be maintained in culture for > 6-8 passages corresponding to a period of 1-2 months.

  Related Links and Documents

  • Human Cardiac Myocytes
  • Myocyte Growth Medium
• Can I mix the SkMC Supplement with the Skeletal Muscle Cell Basal Medium and freeze some down for later use? If so, how long can it remain frozen?

  The PromoCell Basal Media must be stored between 4-8°C and should not be frozen, as this can lead to precipitations. The same is true after addition of the supplements: the complete medium has to be kept at 4-8°C.
  If you prefer to make up smaller volumes of complete medium, you can aliquot the Supplement Mix and refreeze those aliquots at -20°C until use. This way you can extend the period in which you can use the supplemented media.

  Related Links and Documents

  • SkMC Growth Medium
• What is the source of PromoCell’s Normal Human Follicle Dermal Papilla Cells? For how long can they be maintained in culture?

  HFDPC are isolated from the hair papilla of normal human scalp hair follicles. Hair papilla in the adult hair follicle play a crucial role in the dermal-epidermal interactions that control hair production and in hair growth cycle events. The follicle dermal cells are cryopreserved at second passage and can be cultured for at least 10 population doublings when using PromoCell Follicle Dermal Papilla Cell Growth Medium (Cat. C-26501). Typical population doubling times are between 20-36 hrs.
  The recommended seeding density after thawing/trypsinization is 5,000-10,000 cells/cm². Using 1:4 splits, you can perform 4-5 passages with the cells.

  Related Links and Documents

  • HFDPC
  • Follicle Dermal Papilla Cell Medium
  • Dermal papilla
• What is the basis formula for PromoCell’s Osteoblast Basal Medium (C-27010)?

  PromoCell’s Osteoblast Basal Medium is an optimized media formulation developed for human osteoblast culture. The exact composition is proprietary.

  Related Links and Documents

  • Osteoblast Basal Medium
• What is the approximate cell density of HFDPC (C-12071) at subconfluence?

  Typical cell densities are between 32,000 – 40,000 cells/cm² (approx. 800,000 – 1 million cells per T25-flask).

  Related Links and Documents

  • HFDPC
• How many osteoblasts will be in the flask when I purchase a proliferating culture (C-12760)?

  If you purchase a proliferating culture of human osteoblasts (C-12760), there will be > 500,000 cells in the TC-flask. Once the culture is subconfluent, you will count between 750,000 – 1.1 million cells/T25 (depending on the cell lot).

  Related Links and Documents

  • HOB
• Does the Osteoblast Growth Medium support osteoblast proliferation or differentiation? Does the medium contain any recombinant growth factors?

  Our Osteoblast Growth Medium (C-27001) consists of the Basal Medium supplemented with 10% (v/v) FCS, but with no recombinant growth factors. The medium primarily supports the proliferative capacity of normal human osteoblasts. It does not contain osteogenic factors (like dexamethasone and beta-glycerophosphate) that promote differentiation as many users test their own chemical compounds (growth factors, hormones), or examine the effects of physical strain or sheer stress on the differentiated functions. The Osteoblast Growth Medium is well-suited as the basis for these applications and can be supplemented with further growth factors if necessary. To specifically induce mineralization, PromoCell supply Osteoblast Mineralization Medium (C-27020).

  Related Links and Documents

  • Osteoblast Media
• Is it possible to split differentiated adipocytes in order to perform more experiments?

  Generally, you can split differentiated adipocytes. But these cells lose their ability to proliferate after differentiation and you can’t expand the culture any more. Therefore it is recommended to plate the cells into the needed vessels (e.g. multiwell plates) prior to induction of differentiation so that trypsinization isn’t necessary.

  Related Links and Documents

  • HWP