Technical Library

Recent Entries in Technical Library

• Why are PromoCell products “for in vitro research use only”?

  The basis for the intended use of our products is defined in our Terms & Conditions under the chapter “Use of Goods”.

   

• How long do I need to activate my macrophages? Can I stop adding activation factors after 24 hours?

  The activation of macrophages as such is complete after 24 hrs. However, to maintain the activation status over a longer period of time (i.e., several days), fresh activation factors should be added with every medium change.

  Related Links and Documents

  • Application Note: Assay-ready adherent cultures of fully functional human primary macrophages
  • Macrophage Cell Culture
• Once thawed and opened, does Aquaguard-1 need to be refrigerated or can it stay at room temperature? Can I pre-dilute it in water and leave it at room temperature?
  1. We recommend storing Aquaguard-1 at 4°C after thawing. At this temperature it is stable for up to one month. For long-term storage, we recommend a storage temperature of -20°C. Aquaguard-1 can be frozen and thawed up to 5 times without any problems.
     It is also stable for some time at room temperature, but we do not have sufficient data to guarantee a specific time period and therefore do not recommend this.
  2. As mentioned above, we cannot guarantee exactly how long Aquaguard-1 will be stable at room temperature, and therefore do not recommend it. Also, it would be important to store it in the dark, as constant sunlight/UV light will affect its stability.

  Related Links and Documents

  • Aquaguard-1 (incubator)
• Can the matrix in the Angiogenesis Assay Kit (PK-CA577-K905) be left at RT?

  The matrix should always be kept on ice and multiple freeze/thaw should be avoided.

  Related Links and Documents

  • Angiogenesis Assay Kit
• Can the Cytokine Mix E for the Hematopoietic Progenitor Expansion Medium XF (C-39891; 5 ml) be safely aliquoted?

  Yes, it is possible to aliquot the 5 ml Cytokine Mix E (C-39891) into 5 x 1 ml.

  Related Links and Documents

  • Cytokine Mix E
• What is the difference between PromoCell NHEK and NHEK GM3?

  The NHEK (primary human keratinocytes) are isolated in our serum-free Keratinocyte Growth Medium 2 (C-20011), the NHEK GM3 in our improved serum-free and BPE-free Keratinocyte Growth Medium 3 (C-20021). Both, NHEK and NHEK GM3 are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin.

  Related Links and Documents

  • NHEK
  • NHEK GM3
• Will my NHEK (isolated in PromoCell Keratinocyte Growth Medium 2) grow in PromoCell Keratinocyte Growth Medium 3?

  Yes, the NHEK-GM2 (C-12001, C-12003, C-12005, C-12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C-20021). Using the protocol with the fixed intervals, they grow slightly faster than in Keratinocyte GM2 and proliferate for > 15 PDs.
  Conversely, NHEK-GM3 (C-12011, C-12013, C-12015, C-12016) also grow in the existing Keratinocyte Growth Medium 2 (C-20011). When using the classical subcultivation protocol (density > 70% – 90%), they grow slightly slower compared to Keratinocyte GM3, but also reach > 15 PDs.

  Related Links and Documents

  • Keratinocyte Cell Culture
• Will the NHEM.f (C-12400) and NHEM.f M2 (C-12402) also grow in PromoCell Melanocyte Growth Medium M3?

  Yes, NHEM.f and NHEM.f M2 also grow in our optimized Melanocyte Growth Medium M3 (C24310) and can achieve > 15 population doublings.

  Related Links and Documents

  • Melanocyte Cell Culture
• I have a question regarding your Alanine Aminotransferase Activity Kit (PK-CA577-K752). What is the activity of the ALT Positive Control?

  The activity of the positive control is not determined. However, we can give you the OD value for the positive control from a specific lot. Please feel free to contact and share with us the lot number if you would like to see the expected OD value.

  Related Links and Documents

  • Alanine Aminotransferase (ALT/SGPT) Activity Assay Kit
• Which human AB serum does PromoCell recommend to generate M0 macrophages?

  We recommend to use Human Serum AB „off-the-clot”.

  Related Links and Documents

  • Macrophage Base Medium DXF
  • Application Note: Convenient large-scale differentiation