Technical Library

Recent Entries in Technical Library

• We use Macrophage Generation Media from PromoCell to get differentiated macrophages from peripheral blood monocytes. The monocytes are isolated by culturing PBMCs in Monocyte Attachment Media (also from PromoCell) for 1.5 h. Would it be ok if the attachment step is done overnight, since we receive the blood later in the day which makes it difficult to process on the same day?

  It is not recommended to leave the blood cells in the Monocyte Attachment Medium for longer than 1.5-2 hrs. The medium was developed for (short-term) attachment of the monocytes and does not provide nutrients for a longer time period. Leaving the cells in Monocyte Attachment Medium for a longer time or even overnight will induce apoptosis and lead to the loss of the cells.
  If necessary, you can reduce the incubation time to 1 hr. In this case, it is advisable to equilibrate the media in the incubator before so that you can immediately and directly add the appropriate amount of PBMC suspension.

  Related Links and Documents

  • Application Note: Differentiation of M1-or M2-Macrophages from PBMC/Monocytes
  • Monocyte Attachment Medium
• Does PromoCell recommend a particular manufacturer or brand of tissue culture plastic to grow the primary cells or can I use any supplier on the market?

  Our customers have successfully used TC flasks and dishes from all the leading cell culture plastic suppliers to grow PromoCell’s primary human cells. We do not have any knowledge whether the dishes from local TC plastic suppliers work in the same way. We recommend to first test whether these brands provide the same good performance as the plastic of the leading manufacturers.

• After differentiation of human MSC into mature adipocytes – what medium do I have to use to culture those cells?

  Differentiation of hMSC into mature adipocytes takes approx. 2 weeks. You can keep the adipocytes for up to 3 weeks in the MSC Adipogenic Differentiation Medium. After 3 weeks we recommend to switch to PromoCell’s Adipocyte Nutrition Medium (C-27438).

  Related Links and Documents

  • Mesenchymal Stem Cells
  • MSC Adipogenic Differentiation Medium 2
  • Adipocyte Nutrition Medium
  • Application Note: Adipogenic Differentiation and Analysis of MSC
• What is the composition of Melanocyte Growth Medium M2 SupplementMix (C-39420)?

  Supplement Mix for Melanocyte Growth Medium M2 (C-39420) is free of serum and doesn’t contain any PMA. The qualitiative and quantitative composition is proprietary.

  Related Links and Documents

  • Melanocyte Growth Medium M2
• What is the source of PromoCell’s Normal Human Follicle Dermal Papilla Cells? For how long can they be maintained in culture?

  HFDPC are isolated from the hair papilla of normal human scalp hair follicles. Hair papilla in the adult hair follicle play a crucial role in the dermal-epidermal interactions that control hair production and in hair growth cycle events. The follicle dermal cells are cryopreserved at second passage and can be cultured for at least 10 population doublings when using PromoCell Follicle Dermal Papilla Cell Growth Medium (Cat. C-26501). Typical population doubling times are between 20-36 hrs.
  The recommended seeding density after thawing/trypsinization is 5,000-10,000 cells/cm². Using 1:4 splits, you can perform 4-5 passages with the cells.

  Related Links and Documents

  • HFDPC
  • Follicle Dermal Papilla Cell Medium
  • Dermal papilla
• Do PromoCell HDMEC exhibit migration and tube formation as a response to angiogenic stimuli?

  In principle, all microvascular endothelial cells should be able to migrate, proliferate and form tubes or sprouts in an appropriate assay after angiogenic stimulation. As this is not part of our routine quality control procedure, we cannot tell for sure whether all cell lots will respond to angiogenic stimuli. However PromoCell also supplies HDMEC pre-screened (C-12215) that are especially tested for a positive VEGF response.

  Related Links and Documents

  • HDMEC pre-screened
• How should I store the PromoCell media and supplements?

  Upon arrival, the basal media should be stored between 4°C and 8°C, the supplements at -20°C.

• What can pericytes differentiate into?

  Pericytes have been shown to differentiate e.g. into adipocytes, osteoblasts, chondrocytes, fibroblasts/myofibroblasts, vascular smooth muscle cells, and phagocytes.

  Related Links and Documents

  • hPC-PL
  • Diaz-Flores et al.
• How many passages can I perform with PromoCell HUVECs?

  PromoCell guarantee > 15 PD for their HUVEC. The number of passages that can be performed, depends on the dilution factor used during subculture. If you split the cells 1:4, they can perform about 2 population doublings per passage which means that they can be cultured for at least 6-8 passages.

  Related Links and Documents

  • HUVEC
• Can I trypsinize the PromoCell Normal Human Cells at 37°C?

  PromoCell recommend to trypsinize all Normal Human Cells at room temperature and to monitor the detachment under the microscope. Prolonged trypsinization at 37°C can lead to irreversible damage of the cells.

  Related Links and Documents

  • Subcultivation of primary cells