Technical Library

Recent Entries in Technical Library

• Will the NHEM.f (C-12400) and NHEM.f M2 (C-12402) also grow in PromoCell Melanocyte Growth Medium M3?

  Yes, NHEM.f and NHEM.f M2 also grow in our optimized Melanocyte Growth Medium M3 (C24310) and can achieve > 15 population doublings.

  Related Links and Documents

  • Melanocyte Cell Culture
• I have a question regarding your Alanine Aminotransferase Activity Kit (PK-CA577-K752). What is the activity of the ALT Positive Control?

  The activity of the positive control is not determined. However, we can give you the OD value for the positive control from a specific lot. Please feel free to contact and share with us the lot number if you would like to see the expected OD value.

  Related Links and Documents

  • Alanine Aminotransferase (ALT/SGPT) Activity Assay Kit
• Following your protocol in a 96-well plate, how thick is the resulting Alginate Hydrogel matrix usually? And what are the maximum dimensions of an alginate hydrogel which still allow a sufficient exchange of nutrients and oxygen?

  50 µl of Alginate Hydrogel 3D Cell Culture Matrix per well (96-well plate format) is what is recommended. We have not measured to see what the exact thickness of this is. But this is enough to provide sufficient exchange of nutrients and oxygen. Thicker matrices may not be advisable but you may try.

   

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  • Alginate Hydrogel 3D Cell Culture Matrix
• Does the BME 3D Cell Culture Matrix (PK-CA577-K518-5) contain laminin-1?

  The BME (Basement Membrane Matrix; animal-based) is rich in extracellular matrix proteins, including laminin (a major component), Collagen IV, heparin sulfate proteoglycans and a number of growth factors.

   

  Related Links and Documents

  • BME 3D Cell Culture Matrix
• According to literature, the NOS seems to need 3 or 5 cofactors to function, but the Nitric Oxide Synthase Activity Assay Kit contains only two, i.e. NOS Cofactor 1 and 2.

  The Cofactor Solutions are not a single reagent solution, but a mix of different cofactors needed for NOS reaction to proceed. Unfortunately, further information is proprietary and therefore cannot be disclosed.

  Related Links and Documents

  • Nitric Oxide Synthase (NOS) Activity Assay Kit I
• I have a question about the human fibronectin included in your Cell Invasion Assay Kit (PK-CA577-K925): What is the origin of the FN?

  The fibronectin is derived from human plasma and is produced in an animal-component free process.

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  • Cell Invasion Assay Kits
• How much whole blood is required for 3 ml of the Lymphocyte Separation Medium in a 15 ml conical tube?

  In general the 15 ml tubes are filled with 3 ml Lymphocyte Separation Medium 1077 and the 50 ml conical tubes with 15 ml Lymphocyte Separation Medium.

  For the 15 ml tubes, it is recommended to use 3-8 ml, for the 50 ml tubes 15 – 30 ml of diluted* whole blood.

  *1:2 -1:3 in PBS

  Related Links and Documents

  • Lymphocyte Separation Medium 1077
• How do I get maximum expansion of the undifferentiated CD34+ cells? Is there a maximum cell density?

  The following is what we do here in our research lab for maximum expansion of the undifferentiated cells:
  – Seed the vial of cells at a density of 5,000 cells/ml as per PromoCell protocol (see Manual of human CD34⁺ progenitor cells).
  – Use our Hematopoietic Progenitor Cell Expansion Medium XF (C-28021) supplemented with our Cytokine Mix E.
  – Allow the cells to grow for one week. It may take 4-5 days for the cells to noticeably proliferate, but they will grow rapidly after this.
  – After the first week, change 2/3 of the media and culture for a second week.
  You should see up to a 200-fold expansion of the cells within this time. Cell density is not the major concern when expanding the progenitors. The media used and time in culture are more important factors. Some researchers expand the progenitors out a third week but we don’t recommend this for the health of the cells.

  Related Links and Documents

  • Hematopoietic Stem Cell Culture
• I have two questions regarding the PCR Mycoplasma Test Kit I/C. It is recommended to use a 1.5% agarose gel for the analysis. Would it also be possible to use a 2% or 3% pre-cast agarose gel? And do you recommend to use a DNA ladder?
  1. It is recommended to use a 1.5% agarose gel for the analysis of the amplified DNA. Theoretically, it is also possible to use a 2% or 3% pre-cast agarose gel as long as it is running for a sufficient distance to separate the bands at ∼270 bp (positive control and mycoplasma- positive samples) and 479 bp (internal control).
  2. A DNA ladder is not mandatory but is recommended to correctly identify all bands. We recommend using a 100 bp ladder, but a 250 bp ladder or similar, which allows you to identify the 270 and 479 bp bands is also suitable.

  Related Links and Documents

  • PCR Mycoplasma Test Kit I/C
• Which human AB serum does PromoCell recommend to generate M0 macrophages?

  We recommend to use Human Serum AB „off-the-clot”.

  Related Links and Documents

  • Macrophage Base Medium DXF
  • Application Note: Convenient large-scale differentiation