Technical Library

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• I have a question concerning your 3D Cell Culture Matrix BME Kit. I need to load 50 μl of BME per well of a pre-cooled 96-well plate. Even if no air bubbles are created by pipetting, some air bubbles are appearing when the BME is gelling. How can I avoid this?

  Please thaw the BME Matrix (PK-CA577-K518/PK-CA577-K518-5) on ice and keep on ice until you need it. BME when warmed to 37 C will solidify. Please do not do that.
  You can try quickly spinning the BME down to get rid of the microbubbles – no sonication either. Please handle gently when thawing without much shaking or moving.

   

  Related Links and Documents

  • 3D Cell Culture Matrix Kit (BME)
• Can the matrix in the Angiogenesis Assay Kit (PK-CA577-K905) be left at RT?

  The matrix should always be kept on ice and multiple freeze/thaw should be avoided.

  Related Links and Documents

  • Angiogenesis Assay Kit
• Can the Cytokine Mix E for the Hematopoietic Progenitor Expansion Medium XF (C-39891; 5 ml) be safely aliquoted?

  Yes, it is possible to aliquot the 5 ml Cytokine Mix E (C-39891) into 5 x 1 ml.

  Related Links and Documents

  • Cytokine Mix E
• What is the difference between PromoCell NHEK and NHEK GM3?

  The NHEK (primary human keratinocytes) are isolated in our serum-free Keratinocyte Growth Medium 2 (C-20011), the NHEK GM3 in our improved serum-free and BPE-free Keratinocyte Growth Medium 3 (C-20021). Both, NHEK and NHEK GM3 are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin.

  Related Links and Documents

  • NHEK
  • NHEK GM3
• How long can I store the reconstituted VEGF-165 at -20°C?

  Storage depends somewhat on the concentration of the solution. We set the concentration of the solution we need for our proliferation experiments to 50 µg/ml.
  The mixtures for the experiments are then prepared from this stock solution. The stock solution can easily be stored for > 1 year at -20° C.

  Related Links and Documents

  • VEGF-165, human, recombinant (E. coli)
• Will my NHEK (isolated in PromoCell Keratinocyte Growth Medium 2) grow in PromoCell Keratinocyte Growth Medium 3?

  Yes, the NHEK-GM2 (C-12001, C-12003, C-12005, C-12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C-20021). Using the protocol with the fixed intervals, they grow slightly faster than in Keratinocyte GM2 and proliferate for > 15 PDs.
  Conversely, NHEK-GM3 (C-12011, C-12013, C-12015, C-12016) also grow in the existing Keratinocyte Growth Medium 2 (C-20011). When using the classical subcultivation protocol (density > 70% – 90%), they grow slightly slower compared to Keratinocyte GM3, but also reach > 15 PDs.

  Related Links and Documents

  • Keratinocyte Cell Culture
• Will the NHEM.f (C-12400) and NHEM.f M2 (C-12402) also grow in PromoCell Melanocyte Growth Medium M3?

  Yes, NHEM.f and NHEM.f M2 also grow in our optimized Melanocyte Growth Medium M3 (C24310) and can achieve > 15 population doublings.

  Related Links and Documents

  • Melanocyte Cell Culture
• I have a question regarding your Alanine Aminotransferase Activity Kit (PK-CA577-K752). What is the activity of the ALT Positive Control?

  The activity of the positive control is not determined. However, we can give you the OD value for the positive control from a specific lot. Please feel free to contact and share with us the lot number if you would like to see the expected OD value.

  Related Links and Documents

  • Alanine Aminotransferase (ALT/SGPT) Activity Assay Kit
• Following your protocol in a 96-well plate, how thick is the resulting Alginate Hydrogel matrix usually? And what are the maximum dimensions of an alginate hydrogel which still allow a sufficient exchange of nutrients and oxygen?

  50 µl of Alginate Hydrogel 3D Cell Culture Matrix per well (96-well plate format) is what is recommended. We have not measured to see what the exact thickness of this is. But this is enough to provide sufficient exchange of nutrients and oxygen. Thicker matrices may not be advisable but you may try.

   

  Related Links and Documents

  • Alginate Hydrogel 3D Cell Culture Matrix
• Does the BME 3D Cell Culture Matrix (PK-CA577-K518-5) contain laminin-1?

  The BME (Basement Membrane Matrix; animal-based) is rich in extracellular matrix proteins, including laminin (a major component), Collagen IV, heparin sulfate proteoglycans and a number of growth factors.

   

  Related Links and Documents

  • BME 3D Cell Culture Matrix