Cancer Cell Line Medium XF

Serum-free and xeno-free medium for the long-term cultivation of established cancer cell lines.

Cancer Cell Line Medium XF
C-28077
250 ml
$ 140.00

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Cancer Cell Line Medium XF

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Key benefits:

  • Suitable for long-term routine culture of adherently growing established human cancer cell lines
  • Compatible with most commonly used human cancer cell lines
  • Serum- and xeno-free
  • Standardized and reproducible system

Components:

  • Cancer Cell Basal Medium
  • Cancer Cell SupplementMix

Our Cancer Cell Line Medium XF is the working horse of the cancer cell culture toolbox. It allows you to cultivate or co-cultivate a range of different cancer cells, cancer-associated cells, and non-cancer cells such as immune or stromal cells. This rich growth medium supports the long-term cultivation of cancer cells over many passages.

You can use the Cancer Cell Line Medium XF as a standalone to expand and maintain a cancer cell population in a standardized and reproducible environment. Or you can use it in combination with the Primary Cancer Cell Culture System (PCCS) and the 3D Tumorsphere Medium XF, e.g., to establish cancer cell lines from tumor biopsies.

Cancer cell Line Medium XF In Cancer Media Tool Box

 

Due to the flexible nature of our Cancer Cell Line Medium XF, you can use it to support a broad range of cancer cell lines or cancer cells from tumor biopsies while maintaining the tumor-initiating cancer stem cells (CSCs).

Cancer Cell Line Medium XF is serum-free and xeno-free and provides an environment free from non-defined materials, such as fetal calf serum, extracts, or hydrolysates. Cancer Cell Line Medium XF also shows very low lot-to-lot variability.

These properties provide the ideal culture environment to be used for other applications such as co-cultivation of cancer cells and immune cells for cell therapy development like CAR-T cells. The serum- and xeno-free formulation supports the expansion of extracellular vesicle (EV)-producing cancer cells in an EV-free environment.

Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin and vitronectin coating.

Cell types tested for serial passage with our Cancer Cell Line Medium XF:

Tissue Tested cell lines Cell lines origin Remarks
Brain BV2 Immortalized murine primary microglial cells Coat with Fibronectin:1 µg/cm2
Breast HCC38 Human breast ductal carcinoma cell line Coat with collagen 6 µg/cm2
Breast HCC1143 Human ductal carcinoma cell line Coat with collagen 6 µg/cm2
Breast HCC1937 Human ductal carcinoma cell line Coat with collagen 6 µg/cm2
Breast MCF-7 Pleural effusion of metastatic human breast adenocarcinoma Coat with Fibronectin:1 µg/cm2
Colon HT-29 Human colon adenocarcinoma Coat with Vitronectin:0.5 µg/cm2
Connective tissue HT1080 Human fibrosarcoma Coat with Fibronectin:1 µg/cm2
Liver HepG2 Hepatocellular carcinoma of the human liver Coat with Vitronectin:0.5 µg/cm2
Lung A-549 Human lung carcinoma Coat with Vitronectin:0.5 µg/cm2
Prostate LNCaP Lymph node metastasis of human prostate adenocarcinoma 3D culture in C-28070 is recommended
Peripheral blood BDCM B lymphoblast cell line No coating required
Peripheral blood HCC38 BL EBV transformed B lymphoblast cell line No coating required
Peripheral blood MV-4-11 Human acute myelogenous leukemia (suspension) No coating required
Bone marrow KG-1 Human acute myelogenous leukemia (suspension) No coating required
Kidney ACHN Human Renal Cell Carcinoma Coat with Fibronectin:1 µg/cm2
Brain A172 Human Glioblastoma Coat with Fibronectin:1 µg/cm2
Skeletal muscle C2C12 Mouse Myoblasts Coat with Fibronectin:1 µg/cm2
Skin B16-F10 Mouse Melanoma Coat with Fibronectin:1 µg/cm2
Abelson murine leukemia virus-induced tumor RAW264.7 Mouse Macrophage Coat with Fibronectin:1 µg/cm2
Brain, neuroectodermal Neuro-2a Mouse Neuroblastoma Coat with Fibronectin:1 µg/cm2
Growth Curves
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Growth Curves
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
CCLM Cancer Cell Line Examples
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
CCLM Cancer Cell Line Examples
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.

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