Technical Library

Recent Entries in Technical Library

• Following the recommended seeding density for your M1 macrophages (100,000 cells per cm2), I would need too many cells to have a complete 96- or 384-well plate. This is too expensive for me, can I reduce the number of cells per cm2?

  The recommended seeding density with 100,000 cells per cm² is needed for a confluent cell layer as the cells do not proliferate.
  However, you can reduce the seeding density by the factor 3 to 5 and the macrophages are still viable.

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  • Macrophage Cell Culture
• Do you recommend a certain negative control for the activated macrophages to compare the cytokine release with?

  Even non-activated macrophages do release a certain amount of cytokines. Furthermore, you would have to be sure that the release of a certain cytokine is a direct consequence of the activation. Therefore, we do not think it is possible to have a general negative control for the cytokine release.

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  • Macrophage Cell Culture
• Does PromoCell have any data regarding the cytokine profile and different markers of the M1/M2 macrophages after activation?

  No, we do not provide data about the cytokine profile of our M1/M2 macrophages after activation and we do not provide any further data beyond the scope of our quality control.

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  • Macrophage Cell Culture
• Which cytokine concentrations does PromoCell recommend for activating M1/M2 macrophages?

  You may find all information regarding the activation and the cytokine concentrations in table 1 (page 5) of our Application Note.

  Related Links and Documents

  • Application Note
  • Macrophage Cell Culture
• Did PromoCell try plating the cryo-macrophages in 96-well plates?

  We did not plate the cryo-macrophages in 96-well plates. However, we heard from other customers that they have successfully used our macrophages in this kind of plate.

  Related Links and Documents

  • Macrophage Cell Culture
• Is the Aromatase Assay Buffer compatible with a classical BCA protein quantification kit (e.g. Pierce™ BCA Protein Assay Kit)?

  The assay buffer of our Aromatase Activity Assay Kit (PK-CA577-K983) has 50 mM potassium phosphate and may not be compatible with BCA. But it is compatible with Bradford assay.

  Related Links and Documents

  • Aromatase (CYP19A) Activity Assay Kit
• What MATra reagent does PromoCell recommend for the transfection of vascular Smooth Muscle Cells?

  Bovine carotid artery smooth muscle cells have been successfully transfected using MATra-A.

  Related Links and Documents

  • Magnet-Assisted Transfection
• What’s the difference between PromoFectin (PK-CT-2000-100) and PromoFectin-siRNA (PK-CT-2000-RNA-200)?

  Both, PromoFectin and PromoFectin-siRNA, are non-liposomal transfection reagents.

  • Our (broad-range) PromoFectin can be used to transfect primary cells and cell lines with DNA or RNA.
  • PromoFectin-siRNA was developed to specifically deliver siRNA into a wide variety of cell types.

  Related Links and Documents

  • PromoFectin reagents
• Can I spray the Spore-EX Spray on plexiglass surfaces without damaging it?

  This spray is compatible with plastic/plexiglass. However it will leave residue as it is not an alcohol based formulation, so you may need to wipe dry with a paper towel.

  Related Links and Documents

  • Spore-EX Spray
• I received 3 DetachKits yesterday. Some of the tubes have different colors, although they all come from the same batch.

  The components of the PromoCell DetachKit may occasionally arrive with a non-uniform color appearance. This phenomenon is known to PromoCell’s Quality Assurance Dept.
  It is reversible and has no influence on the quality of the product.

  Related Links and Documents

  • PromoCello DetachKit