Have a question about one of our products? Check out our technical library for recently asked questions from other scientists around the world.
Technical Library
Recent Entries in Technical Library
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Following the recommended seeding density for your M1 macrophages (100,000 cells per cm2), I would need too many cells to have a complete 96- or 384-well plate. This is too expensive for me, can I reduce the number of cells per cm2?
The recommended seeding density with 100,000 cells per cm2 is needed for a confluent cell layer as the cells do not proliferate.
However, you can reduce the seeding density by the factor 3 to 5 and the macrophages are still viable.Related Links and Documents
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Do you recommend a certain negative control for the activated macrophages to compare the cytokine release with?
Even non-activated macrophages do release a certain amount of cytokines. Furthermore, you would have to be sure that the release of a certain cytokine is a direct consequence of the activation. Therefore, we do not think it is possible to have a general negative control for the cytokine release.
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Does PromoCell have any data regarding the cytokine profile and different markers of the M1/M2 macrophages after activation?
No, we do not provide data about the cytokine profile of our M1/M2 macrophages after activation and we do not provide any further data beyond the scope of our quality control.
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Which cytokine concentrations does PromoCell recommend for activating M1/M2 macrophages?
You may find all information regarding the activation and the cytokine concentrations in table 1 (page 5) of our Application Note.
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Did PromoCell try plating the cryo-macrophages in 96-well plates?
We did not plate the cryo-macrophages in 96-well plates. However, we heard from other customers that they have successfully used our macrophages in this kind of plate.
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Is the Aromatase Assay Buffer compatible with a classical BCA protein quantification kit (e.g. Pierce™ BCA Protein Assay Kit)?
The assay buffer of our Aromatase Activity Assay Kit (PK-CA577-K983) has 50 mM potassium phosphate and may not be compatible with BCA. But it is compatible with Bradford assay.
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What MATra reagent does PromoCell recommend for the transfection of vascular Smooth Muscle Cells?
Bovine carotid artery smooth muscle cells have been successfully transfected using MATra-A.
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What’s the difference between PromoFectin (PK-CT-2000-100) and PromoFectin-siRNA (PK-CT-2000-RNA-200)?
Both, PromoFectin and PromoFectin-siRNA, are non-liposomal transfection reagents.
- Our (broad-range) PromoFectin can be used to transfect primary cells and cell lines with DNA or RNA.
- PromoFectin-siRNA was developed to specifically deliver siRNA into a wide variety of cell types.
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Can I spray the Spore-EX Spray on plexiglass surfaces without damaging it?
This spray is compatible with plastic/plexiglass. However it will leave residue as it is not an alcohol based formulation, so you may need to wipe dry with a paper towel.
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I received 3 DetachKits yesterday. Some of the tubes have different colors, although they all come from the same batch.
The components of the PromoCell DetachKit may occasionally arrive with a non-uniform color appearance. This phenomenon is known to PromoCell’s Quality Assurance Dept.
It is reversible and has no influence on the quality of the product.Related Links and Documents
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