Technical Library

Recent Entries in Technical Library

• How can I avoid precipitates when preparing my Mesenchymal Stem Cell Adipogenic Differentiation Medium 2?

  The supplement should be at room temperature when added to the MSC Adipogenic Basal Medium 2. It may also be beneficial to invert the tube a few times to bring precipitates back into solution.

  Please note: It is not recommended to filter the basal medium, supplements, or complete medium, as components that induce or promote differentiation may be removed, resulting in a low differentiation rate when using the medium.

  Related Links and Documents

  • Mesenchymal Stem Cell Adipogenic Differentiation Medium 2
• Are there any differences in the cultivation protocol when cultivating MSCs compared to other cell types?

  Yes, there are a few differences:
  – We recommend replacing the MSC Growth Medium XF (C-28019) 3-4 h after seeding, as opposed to 16-24 hours after seeding for most other cell types/growth media.
  – When MSC Growth Medium XF, MSC Neurogenic Differentiation Medium, MSC Adipogenic Differentiation Medium 2 or MSC Osteogenic Differentiation Medium are used, flasks have to be coated with 10 µg/cm² (human or bovine) fibronectin according to the instruction manual.
  – We strongly recommend using Accutase (C-41310) for cell detachment instead of Trypsin. If Trypsin is used, contact time should not exceed 2 min.

   

  Related Links and Documents

  • Mesenchymal Stem Cell Culture
• After thawing the supplements, I see some precipitation. Is this normal?

  Light flocculation may be seen upon thawing the supplements containing ECGS/heparin or BPE. This does not affect the activity of our media.
  Optionally, the precipitate can be removed by centrifugation under sterile conditions.

  We recommend to thaw the supplements (SupplementMix or SupplementPack) at 15-25°C.

• We are working with PromoCell MSCs and MSC Adipogenic Differentiation Medium. Do you have any information on a comparison between Sudan III and Oil Red O and if Sudan III is more efficient at labeling lipid vesicles?

  You can also use Oil Red O to stain lipid droplets.  At PromoCell, we used to use Oil Red O as well, but switched to Sudan III some time ago for organizational reasons.

   

  Related Links and Documents

  • Application Note: Adipogenic Differentiation and Analysis of MSC
  • Human Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Adipogenic Differentiation Medium 2
• Are PromoCell HPAEC and HPASMC obtained from the proximal or distal part of the pulmonary artery?

  Our HPAEC and HPASMC are isolated from the proximal pulmonary artery.

  Related Links and Documents

  • Human Pulmonary Artery Endothelial Cells
  • Human Pulmonary Artery Smooth Muscle Cells
• Why are PromoCell products “for in vitro research use only”?

  The basis for the intended use of our products is defined in our Terms & Conditions under the chapter “Use of Goods”.

   

• How long do I need to activate my macrophages? Can I stop adding activation factors after 24 hours?

  The activation of macrophages as such is complete after 24 hrs. However, to maintain the activation status over a longer period of time (i.e., several days), fresh activation factors should be added with every medium change.

  Related Links and Documents

  • Application Note: Assay-ready adherent cultures of fully functional human primary macrophages
  • Macrophage Cell Culture
• Can the Cytokine Mix E for the Hematopoietic Progenitor Expansion Medium XF (C-39891; 5 ml) be safely aliquoted?

  Yes, it is possible to aliquot the 5 ml Cytokine Mix E (C-39891) into 5 x 1 ml.

  Related Links and Documents

  • Cytokine Mix E
• What is the difference between PromoCell NHEK and NHEK GM3?

  The NHEK (primary human keratinocytes) are isolated in our serum-free Keratinocyte Growth Medium 2 (C-20011), the NHEK GM3 in our improved serum-free and BPE-free Keratinocyte Growth Medium 3 (C-20021). Both, NHEK and NHEK GM3 are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin.

  Related Links and Documents

  • NHEK
  • NHEK GM3
• Will my NHEK (isolated in PromoCell Keratinocyte Growth Medium 2) grow in PromoCell Keratinocyte Growth Medium 3?

  Yes, the NHEK-GM2 (C-12001, C-12003, C-12005, C-12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C-20021). Using the protocol with the fixed intervals, they grow slightly faster than in Keratinocyte GM2 and proliferate for > 15 PDs.
  Conversely, NHEK-GM3 (C-12011, C-12013, C-12015, C-12016) also grow in the existing Keratinocyte Growth Medium 2 (C-20011). When using the classical subcultivation protocol (density > 70% – 90%), they grow slightly slower compared to Keratinocyte GM3, but also reach > 15 PDs.

  Related Links and Documents

  • Keratinocyte Cell Culture