Technical Library

Recent Entries in Technical Library

• We are culturing human coronary artery smooth muscle cells (HCASMC) from your company. Are these cells obtained from large arteries like LAD or left Circumflex or from small branches of LAD etc. ?

  Our HCASMC (C-12221) are isolated from the large arteries, i.e. from

  • Right coronary artery
  • Left main coronary artery
  • Circumflex coronary artery and
  • Left anterior descending coronary artery.

  Related Links and Documents

  • HCASMC
• How long does it take to differentiate monocytes into mature DCs using PromoCell DC Generation Medium/DC Generation Medium XF?

  It takes 7-8 days to generate fully mature myeloid dendritic cells.

  Related Links and Documents

  • Application Note: Generation of moDCs
  • Dendritic Cell Generation Medium XF
  • Dendritic Cell Generation Medium
• What is the exact localization of PromoCell’s Human Pericytes? How many doublings can they perform in culture?

  Our hPC-PL (C-12980) are isolated from microvessels of the human placenta, from the chorionic villi.
  The number of populations doublings is not determined for each individual cell lot, but in our experience, they can be grown for at least 15 population doublings.

  Related Links and Documents

  • hPC-PL
• My Normal Human Cells didn’t attach after thawing. What could be the reason?

  Poor attachment after thawing can be a result of inappropriate freezing, storing or thawing the cells as well as from inadequate culture conditions (medium, incubator). The attached trouble shooting guide should help you to identify the possible reasons.

  Related Links and Documents

  • Trouble shooting guide: Poor attachment
  • Thawing of primary cells
  • Best Practices in Cell Culture
• Is it possible to split differentiated adipocytes in order to perform more experiments?

  Generally, you can split differentiated adipocytes. But these cells lose their ability to proliferate after differentiation and you can’t expand the culture any more. Therefore it is recommended to plate the cells into the needed vessels (e.g. multiwell plates) prior to induction of differentiation so that trypsinization isn’t necessary.

  Related Links and Documents

  • HWP
• What is the concentration of the trypsin inhibitor in TNS?

  Our TNS solution contains 0.05% (w/v) trypsin inhibitor from soy bean in HepesBSS/0.1% BSA.

  Related Links and Documents

  • TNS
• What is the shelf life of the PromoCell DetachKit/DetachKit-2?

  The three components of DetachKit and DetachKit-2 (HepesBSS, Trypsin/EDTA, TNS) are stable for 1 year from the date of manufacture when stored at -20°C. Once thawed for usage, they should be stored at 4-8°C and can be used for 6 weeks. Please avoid repeated freeze/thaw cycles.

  Related Links and Documents

  • DetachKit
  • DetachKit
• I would like to know the number of population doublings for PromoCell mononuclear cells (C-12907/C-12901) when grown in Mononuclear Cell Medium (C-28030).

  Mononuclear cells are mostly used in immunology, infection biology, hematology and cancer research to study subpopulations of blood cells.
  Our Mononuclear Cell Medium (C-28030) is intended for short-term maintenance (up to 48 hrs) of the thawed hMNC before you proceed with your experiments. The number of PDs will depend on the subsequent cell culture conditions and is not determined by PromoCell.
  Please note: Depending on the conditions, the ratio of the subpopulations will gradually change, as the different blood cell types behave in different manners. Researchers normally start soon after thawing to either select the cell type of their interest (e.g. hematopoietic cells, endothelial progenitor cells) or perform experiments with all populations of hMNCs (e.g. to study effects on toxicity, viability or metabolism).

  Related Links and Documents

  • hMNC
  • Mononuclear Cell Medium
• How can I calculate the population doubling time?

  The population doubling time or generation time (t_g) is usually calculated during the logarithmic phase of growth. It specifies the time (t) in hours needed by the culture to double its cell number.
  
  t_g = t / n

  n: number of population doublings

• What is the precise localization for a) HNEpC, b) HTEpC, c) HBEpC, and d) HSAEpC?

  a) HNEpC (Human Nasal Epithelial Cells) are isolated from nasal mucosa
  b) HTEpC (Human Tracheal Epithelial Cells) from the surface epithelium of trachea
  c) HBEpC from the surface epithelium of bronchie, and
  d) HSAEpC (Human Small Airway Epithelial Cells) from the distal portion of the respiratory tract in the 1 mm bronchiole area
  (comprising the cells from bronchioli and alveoli).

  Related Links and Documents

  • Human Small Airway Epithelial Cells