Have a question about one of our products? Check out our technical library for recently asked questions from other scientists around the world.
Technical Library
Recent Entries in Technical Library
-
Can I aliquot the SupplementMix?
Yes, you can aliquot the SupplementMix upon delivery and freeze down 2 or 4 individual aliquots at -20°C. This way, you can prepare smaller volumes (2 x 250 ml or 4 x 125 ml) of complete culture medium and thus extend the time you can use the medium.
-
Why does PromoCell use CD146 as a marker for pericytes?
CD146 (MUC18) is a surface marker, expressed on pericytes, MSCs, and endothelial cells from large vessels (but not microvascular endothelial cells). In combination with CD34 (a marker for endothelial and hematopoietic cells), pericytes can be characterized by FACS analysis as CD146+/CD34–. The absence of CD34 expression makes sure that the cells are not of hematopoietic/endothelial origin. Further markers that have been described for pericytes are NG2, CD90, alpha-SMA, and PDGFR-beta.
Related Links and Documents
-
Are the HDMEC pre-screened (C-12215) derived from juvenile foreskin or from adult skin?
All HDMEC pre-screened lots that we currently have in stock are isolated from foreskin.
If you need pre-screened HDMEC from adult donors, please contact the PromoCell Technical Customer Support.Related Links and Documents
-
Is it possible to perform experimental starvation with PromoCell Normal Human Cells and the PromoCell media?
Yes, but the experimental starvation conditions have to be determined individually for each cell type. Usually, the cells are maintained in basal medium with reduced growth factor concentrations or lower FBS content. The cells need to be in a good condition and the starvation should be kept as short as possible as prolonged serum and/or growth factor deprivation induces apoptosis.
-
At what passage are PromoCell Normal Human Cells upon arrival?
The passage number varies depending on the cell type. Please refer to the Manual for your cells under “Specifications”.
You will also find the information in the Certificate of Analysis (CoA).Related Links and Documents
-
My Normal Human Cells are growing slowly after subculture. What could be the reason?
Slow growth after subculture can be caused by over-trypsinization or other suboptimal culture conditions. Please see attached trouble shooting guide for possible reasons.
Related Links and Documents
-
How should I seed the preadipocytes for optimal differentiation?
Recommended procedure:
– Thaw the vial according to our protocol
– Seed one part of the cells (5,000 cells/cm²) directly into multiwell plates for subsequent differentiation induction; grow in Preadipocyte Growth Medium until they reach 100% confluency, then induce differentiation according to the recommended protocol
– Seed the other part (5,000 cells/cm²) into a TC dish and expand them in Preadipocyte Growth Medium; trypsinize at subconfluence; if necessary divide the cell suspension again into two parts and use one part for differentiation tests (see above), the other part to continue the culture of undifferentiated HWP.
– Differentiation capacity may decline after 1-2 passages in vitro, therefore best perform your differentiation tests at early passages. HWP at higher passages can still be used for studies that require undifferentiated HWP (e.g. proliferation assays).Related Links and Documents
-
What happens if I grow CD34+ or CD133+ progenitors in Hematopoietic Progenitor Cell Expansion Medium XF (C-28021) without Cytokine Mix E (C-39890)?
The cells will die. Hematopoietic Progenitor Cell Expansion Medium XF always must be supplemented with Cytokine Mix E or an appropriate cocktail of cytokines.
Related Links and Documents
-
Why are the cryopreserved macrophages abbreviated as hMDM-GMCSF(-) and hMDM-MCSF(-), respectively? What does the (-) stand for?
- hMDM-GMCSF is the abbreviation for human monocyte-derived macrophages. They are polarized [⇒ differentiated with GM-CSF] but non-activated [⇒ (-)] M1 macrophages.
- hMDM-MCSF is the abbreviation for human monocyte-derived polarized [⇒ differentiated with M-CSF] but non-activated [⇒ (-)] M2 macrophages.
The macrophages can be seeded into all kinds of TC vessels. After plating, they can be maintained as biologically functional adherent cultures for several weeks.Optionally, user-customizable activation of the cells can be performed. For details, please see attached Application Note, page 2, Fig. 3 and page 5, Tab. 1).
Related Links and Documents
-
I differentiated M1 macrophages from self-sourced PBMCs following your differentiating protocol. In the final cell culture, I can see small cells attached to the macrophages which are CD3+ (T-cells). What is the reason for the T-cell contamination after the differentiation?
The reason for the higher number of lymphocytes in the macrophage culture is probably due to an insufficient washing step during the purification of the monocyte via adherence. The 3 washing steps in our protocol are essential to receive a monocyte population of over 90%.
Related Links and Documents
Choose your Region
Please choose your region for an optimized website experience. So we can provide you with the most useful information for your country.
- North America
- Europe
- Asia