Human Mesenchymal Stem Cells (hMSC)
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
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Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized standard and GMP compliant media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.
Our hMSC are harvested from normal human adipose tissue, bone marrow, and umbilical cord matrix (Wharton’s jelly) of individual donors.
The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. Our hMSC show a verified marker expression profile that complies with ISCT* recommendations, providing well characterized cells.
*ISCT (International Society for Cellular Therapy) Cytotherapy (2006) Vol. 8, No. 4, 315-317
- Request our GMP compliant cell culture media for mesenchymal stem cells.
- Our hMSC are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 500.000 viable cells.
- Proliferating: >500.000 viable cells shipped in growth medium (T75 flask).
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
|Recommended plating density||4,000 cells per cm2|
|Passage after thawing||P2|
|Tested Markers||Differentiation capacity to adipogenic, osteogenic and chondrognic cells tested, CD105 positive, CD73 positive, CD90 positive, CD45 negative, CD34 negative, CD14 negative, CD19 negative, HLA-DR negative|
|Guaranteed population doubling||> 10|