Human CD14+ Monocytes (hMoCD14+-PB)

Human CD14 Monocytes isolated from Peripheral Blood
(hMoCD14 -PB) from single donors.

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Selected product Human CD14+ Monocytes (hMoCD14+-PB)

single donor, cryopreserved 10,000,000 cells

single donor, cell pellet in RNAlater 1,000,000 cells

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Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting as antigen-presenting immune cells, they can phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into several different cell types. The most common applications for Monocytes are the in vitro differentiation into monocyte-derived dendritic cells (moDC) and osteoclasts as well as their use as phagocytic immune cells in immunology and infection biology.

CD14 belongs to the family of LPS receptor antigens and is strongly expressed on the majority of Monocytes. Thus, binding of immunomagnetic particles to this antigen is employed for purification of PromoCell CD14 -Monocytes (hMoCD14 -PB) from Mononuclear Cells (MNC).

PromoCell Human CD14 Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14 Monocytes are isolated by positive selection with > 95% purity.

NEW: Our hMoCD14+ -PB are now also available from HLA-typed donors.

Available formats:

  • Cryopreserved: Cryogenic vial containing 10.000.000 viable cells.
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.

Please note: Cryopreserved Human CD14 Monocytes are not suitable for the in vitro generation of macrophages using our Macrophage Generation Media XF. Always use freshly isolated human mononuclear cells or human monocytes for this application.

Recommended plating density 1,000,000 cells per ml
Monocyte Cell Culture
Figure 1. CD14 Monocyte Cell Culture in phase contrast
Monocyte Cell Culture
Figure 1. CD14 Monocyte Cell Culture in phase contrast
Monocyte Cell Culture Cluster Formation
Figure 2. Cluster formation during differentiation of Monocytes into moDC.
Monocyte Cell Culture Cluster Formation
Figure 2. Cluster formation during differentiation of Monocytes into moDC.
Flow cytometry
Figure 3. Flow cytometric analysis of day 8 mature moDCs generated in the PromoCell DC Generation Medium. More than 98% of the cells are negative for CD14.
Flow cytometry
Figure 3. Flow cytometric analysis of day 8 mature moDCs generated in the PromoCell DC Generation Medium. More than 98% of the cells are negative for CD14.

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