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Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting as antigen-presenting immune cells, they can phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into several different cell types. The most common applications for Monocytes are the in vitro differentiation into monocyte-derived dendritic cells (moDC) and osteoclasts as well as their use as phagocytic immune cells in immunology and infection biology.
CD14 belongs to the family of LPS receptor antigens and is strongly expressed on the majority of Monocytes. Thus, binding of immunomagnetic particles to this antigen is employed for purification of PromoCell CD14 -Monocytes (hMoCD14 -PB) from Mononuclear Cells (MNC).
PromoCell Human CD14 Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14 Monocytes are isolated by positive selection with > 95% purity.
NEW: Our hMoCD14+ -PB are now also available from HLA-typed donors.
- Cryopreserved: Cryogenic vial containing 10.000.000 viable cells.
- Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Please note: Cryopreserved Human CD14 Monocytes are not suitable for the in vitro generation of macrophages using our Macrophage Generation Media XF. Always use freshly isolated human mononuclear cells or human monocytes for this application.
|Recommended plating density||1,000,000 cells per ml|