Starting with 3D cell culture

Making use of 3D cell culture can give you access to new and powerful in vitro models that model the in vivo environment. In this overview, you’ll be introduced to the main methods in 3D culture and how best to use them in your research.

You likely know the potential of 3D culture techniques and perhaps you already use 2D monolayer cultures in your research. What you might not know are the benefits and limitations of some of the most popular approaches to working with cells in 3D.

We’ll introduce you to four important methods: spheroids, organoids, air-liquid interface cultures, and magnetic 3D cell culture. Each section will give you an introduction to how that method works and then discuss its common applications and the primary advantages it offers.

There are also practical tools in there. As well as introducing you to these techniques, we’ve offered a list of recommended reagents to help you get started with 3D cell culture as soon as possible.

Ready to add 3D cell culture to your research?

Want to mimic in vivo conditions in vitro? Then try 3D cell culture. Learn how to get started!

To our 3D Cell Culture page

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