You likely know the potential of 3D culture techniques and perhaps you already use 2D monolayer cultures in your research. What you might not know are the benefits and limitations of some of the most popular approaches to working with cells in 3D.
We’ll introduce you to four important methods: spheroids, organoids, air-liquid interface cultures, and magnetic 3D cell culture. Each section will give you an introduction to how that method works and then discuss its common applications and the primary advantages it offers.
There are also practical tools in there. As well as introducing you to these techniques, we’ve offered a list of recommended reagents to help you get started with 3D cell culture as soon as possible.