The Macrophage Detachment Solution (C-41330) directly affects the cell membrane. HSA in the Wash Buffer supports regeneration of the cell membrane and protects the cells during the critical phase directly after detachment from detrimental effects.
Our HCASMC (C-12221) are isolated from the large arteries; i.e. from
Right coronary artery
Left main coronary artery
Circumflex coronary artery and
Left anterior descending coronary artery.
Differentiation of hMSC into mature adipocytes takes approx. 2 weeks. You can keep the adipocytes for up to 3 weeks in the MSC Adipogenic Differentiation Medium. After 3 weeks we recommend to switch to PromoCell's Adipocyte Nutrition Medium (C-27438).
Our hPC-PL (C-12980) are isolated from microvessels of the human placenta; from the chorionic villi.
The number of populations doublings is not determined for each individual cell lot; but in our experience; they can be grown for at least 15 population doublings.
PromoCell Blood and Blood Progenitor Cells are cryopreserved directly after isolation (= P0). They haven't been in culture before freezing.
Poor attachment after thawing can be a result of inappropriate freezing storing or thawing the cells as well as from inadequate culture conditions (medium incubator). The attached trouble shooting guide should help you to identify the possible reasons.
Yes it is possible to transfect our normal human cells. In general primary and normal cells are much harder to transfect than immortalized cell lines. Apart from the cell type successful transfection also depends on the culture's age and density at transfection the vector used the purity of the nucleic acids the composition of the transfection medium and the experimental conditions.
Our HAoAF (C-12380) are isolated from the Adventitia the outer layer of the aorta. The cells have been characterized as fibroblasts by the expression of fibroblast-specific CD90.
HFDPC are isolated from the hair papilla of normal human scalp hair follicles. Hair papilla in the adult hair follicle play a crucial role in the dermal-epidermal interactions that control hair production and in hair growth cycle events. The follicle dermal cells are cryopreserved at second passage and can be cultured for at least 10 population doublings when using PromoCell Follicle Dermal Papilla Cell Growth Medium (Cat. C-26501). Typical population doubling times are between 20-36 hrs.
The recommended seeding density after thawing/trypsinization is 5;000-10;000 cells/cm2. Using 1:4 splits; you can perform 4-5 passages with the cells.
PromoCell's HDLEC are isolated from skin (dermis). When isolated from juvenile donors (C-12216); the exact localization is foreskin. When derived from adult donors (C-12217); the localization depends on the type of surgery; e.g. breast or temple. You can find the information on the exact localization in the Certificate of Analysis.
Our HDLEC are tested to be positive for CD31; podoplanin; and Prox-1 and are delivered in P2. The recommended culture medium is Endothelial Cell Growth Medium MV2 (C-22022).
These cells are frozen at the end of 2nd culture. Thawing and seeding results in passage 2 (3rd culture). We recommend that they be used for differentiation experiments not later than passage 5.
The differentiation potential of hMSC in vitro is reduced with ongoing population doublings; meaning the earlier differentiation is induced; the higher the differentiation rates.
Centrifuge and resuspend in suitable cold freezing medium at a density of 1-4 x 106 cells/ml
Cool down the cells slowly to -80°C (approx. -1°C per min). We recommend to use "Mr. Frosty" from Nalge or "CoolCell" from Biocision; which both provide gradual and controlled cooling rates when placed in a -80°C freezer overnight.
Transfer the vials into liquid nitrogen for long-term storage
