How can I be sure that the cells in my melanocyte culture are melanocytes? Is the medium selective for melanocytes?
After isolation and purification, PromoCell primary human melanocytes are characterized during quality control to confirm their identity.
How melanocyte identity is confirmed
Selectivity of melanocyte media
1. Melanocyte Growth Medium (C‑24010)
How melanocyte identity is confirmed
- Cells are checked for their typical melanocyte morphology
- Expression of the marker Mel‑5 is verified: Mel‑5 is a 75 kDa glycoprotein that is typically expressed by normal human melanocytes
Selectivity of melanocyte media
1. Melanocyte Growth Medium (C‑24010)
- Developed to promote melanocyte growth in vitro
- However, it does not completely inhibit the growth of other cell types, such as normal human epidermal keratinocytes (NHEK) or normal human dermal fibroblasts (NHDF).
- Therefore, it is essential to start with a pure melanocyte culture when using this medium.
- Much more selective for melanocytes
- More effectively suppresses the growth of contaminating cell types
- Provides improved robustness when working with primary melanocyte cultures
Products relevant to this topic
Normal Human Epidermal Melanocytes (NHEM)
Cat No
C-12400
Primary Human Epidermal Melanocytes isolated in PMA-containing Melanocyte Growth Medium from the epidermis of juvenile foreskin.
$651.00
Normal Human Epidermal Melanocytes (NHEM) adult donor, cultured in M3 Medium
Cat No
C-12413
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.
$765.00
Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin, cultured in M3 Medium
Cat No
C-12422
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.
$760.00
Melanocyte Growth Medium (Ready-to-use)
Cat No
C-24010
Cell culture medium for the cultivation of juvenile human melanocytes. Formulation contains phorbol myristate acetate.
$188.00
Melanocyte Growth Medium M3 (Ready-to-use)
Cat No
C-24310
Serum-free and BPE-free cell culture medium for the cultivation of juvenile and adult human melanocytes. Formulation is PMA-free.
$369.00