How can I be sure that the cells in my melanocyte culture are melanocytes? Have I understood it correctly that the medium is selective for melanocytes?

Question

Accepted Answer

After isolation and purification of our primary human melanocytes, the cells are checked during quality control whether they show a typical morphology and whether they express the marker Mel-5. Mel-5 is a 75 kDa glycoprotein usually expressed by normal melanocytes. Our Melanocyte Growth Medium (C-24010) has been developed to promote melanocyte growth in vitro. It does not however, completely block the growth of other cells (such as: NHEK or NHDF).

Therefore, it is important to have a pure melanocyte culture from the very beginning.

In contrast, the Melanocyte Growth Medium M3 (C-24310) is much more selective and represses the growth of contaminating cells much better.

Therefore, it is important to have a pure melanocyte culture from the very beginning.

In contrast, the Melanocyte Growth Medium M3 (C-24310) is much more selective and represses the growth of contaminating cells much better.