How can I be sure that the cells in my melanocyte culture are melanocytes? Is the medium selective for melanocytes?

After isolation and purification, PromoCell primary human melanocytes are characterized during quality control to confirm their identity.
 
How melanocyte identity is confirmed

• Cells are checked for their typical melanocyte morphology
• Expression of the marker Mel‑5 is verified: Mel‑5 is a 75 kDa glycoprotein that is typically expressed by normal human melanocytes

These criteria ensure the identity and purity of the supplied melanocytes.
 
Selectivity of melanocyte media
 
1. Melanocyte Growth Medium (C‑24010)

• Developed to promote melanocyte growth in vitro
• However, it does not completely inhibit the growth of other cell types, such as normal human epidermal keratinocytes (NHEK) or normal human dermal fibroblasts (NHDF).
• Therefore, it is essential to start with a pure melanocyte culture when using this medium. 

2. Melanocyte Growth Medium M3 (C‑24310)

• Much more selective for melanocytes
• More effectively suppresses the growth of contaminating cell types
• Provides improved robustness when working with primary melanocyte cultures