Will NHEK isolated in Keratinocyte Growth Medium 2 grow in Keratinocyte Growth Medium 3?
Yes. Normal Human Epidermal Keratinocytes isolated in Keratinocyte Growth Medium 2 (NHEK‑GM2; C‑12001, C‑12003, C‑12005, C‑12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C‑20021).
When cultured using the protocol with fixed medium‑change intervals, these cells:
When using the classical subcultivation protocol (subculture at 70–90% confluency), these cells:
When cultured using the protocol with fixed medium‑change intervals, these cells:
- grow slightly faster than in Keratinocyte Growth Medium 2, and
- achieve more than 15 population doublings (PD).
When using the classical subcultivation protocol (subculture at 70–90% confluency), these cells:
- grow slightly slower than in Keratinocyte Growth Medium 3, but
- also reach more than 15 population doublings.
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