Will NHEK isolated in Keratinocyte Growth Medium 2 grow in Keratinocyte Growth Medium 3?
Yes. Normal Human Epidermal Keratinocytes isolated in Keratinocyte Growth Medium 2 (NHEK‑GM2; C‑12001, C‑12003, C‑12005, C‑12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C‑20021).
When cultured using the protocol with fixed medium‑change intervals, these cells:
When using the classical subcultivation protocol (subculture at 70–90% confluency), these cells:
When cultured using the protocol with fixed medium‑change intervals, these cells:
- grow slightly faster than in Keratinocyte Growth Medium 2, and
- achieve more than 15 population doublings (PD).
When using the classical subcultivation protocol (subculture at 70–90% confluency), these cells:
- grow slightly slower than in Keratinocyte Growth Medium 3, but
- also reach more than 15 population doublings.
Products relevant to this topic
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
Cat No
C-12001
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, single donor
Cat No
C-12003
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
Cat No
C-12005
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, pooled
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskin isolated in Growth Medium 3, single donor
Cat No
C-12011
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, single donor
Cat No
C-12013
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
Cat No
C-12015
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled
Cat No
C-12016
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.