What population doubling times can be expected for PromoCell Mesenchymal Stem Cells, and how long does it take from seeding to subculture?
When cultured in Mesenchymal Stem Cell Growth Medium 2 (C‑28009), PromoCell human Mesenchymal Stem Cells (hMSC‑BM, hMSC‑AT, hMSC‑UC) typically show population doubling times of 20- 30 hours. This value represents the mean population doubling time, including the lag phase, measured over 10 population doublings. The Quality Control specification is ≤ 30 hours.
From seeding to subculture: If hMSCs are seeded at a density of 4,000 cells/cm²:
From seeding to subculture: If hMSCs are seeded at a density of 4,000 cells/cm²:
- Cells usually reach subconfluency within 4-7 days
- At this point, they are ready for subculturing
- donor source
- passage number
- handling and culture conditions
Products relevant to this topic
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