What population doubling times can be expected for PromoCell Mesenchymal Stem Cells, and how long does it take from seeding to subculture?
When cultured in Mesenchymal Stem Cell Growth Medium 2 (C‑28009), PromoCell human Mesenchymal Stem Cells (hMSC‑BM, hMSC‑AT, hMSC‑UC) typically show population doubling times of 20- 30 hours. This value represents the mean population doubling time, including the lag phase, measured over 10 population doublings. The Quality Control specification is ≤ 30 hours.
From seeding to subculture: If hMSCs are seeded at a density of 4,000 cells/cm²:
From seeding to subculture: If hMSCs are seeded at a density of 4,000 cells/cm²:
- Cells usually reach subconfluency within 4-7 days
- At this point, they are ready for subculturing
- donor source
- passage number
- handling and culture conditions
Products relevant to this topic
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
Cat No
C-12971
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
Cat No
C-12977
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Mesenchymal Stem Cell Growth Medium 2
Cat No
C-28009
Low-serum cell growth medium for mesenchymal stems cells.