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Skeletal Muscle Differentiation Medium
Defined and animal-component free cell culture medium for differentiation of skeletal muscle cells.
Skeletal Muscle Cell Differentiation Medium (Ready-to-use)
The Skeletal Muscle Cell Differentiation Medium is a defined and animal-component free medium developed to induce the fusion of Human Skeletal Muscle Cells (SkMC) to myotubes with typical multinucleated syncytia.
Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine, rat, canine, and rabbit skeletal muscle cells. View a list of references where this medium was used with other species and cell types.
Components:
- Differentiation Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- SupplementMix: Contains all media supplements premixed in one vial
Media supplement | Final supplement concentration |
---|---|
Insulin (recombinant human) | 10 μg/ml |
After addition of the SupplementMix or SupplementPack to our basal medium do I have to add FCS you obtain the complete growth medium. No further supplementation with serum or growth factors is required. Please note: Our media do not contain antibiotics. If you wish to use antibiotics you can add penicillin/streptomycin or gentamicin/amphotericin B at standard concentrations. Addition of antibiotics can however reduce the doubling time of the cells up to 30-40%.
The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified; the composition of the supplements is confidential.
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