Technical library
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tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
No, we do not provide data about the cytokine profile of our M1/M2 macrophages after activation and we do not provide any further data beyond the scope of our quality control.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
You may find all information regarding the activation and the cytokine concentrations in table 1 (page 5) of our Application Note.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
We did not plate the cryo-macrophages in 96-well plates. However, we heard from other customers that they have successfully used our macrophages in this kind of plate.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
The components of the PromoCell DetachKit may occasionally arrive with a non-uniform color appearance. This phenomenon is known to PromoCell’s Quality Assurance Dept. It is reversible and has no influence on the quality of the product.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
Scientific findings from different groups, as well as our own results indicate that the presence or absence of α-SMA is not a valid indicator for the composition of a given cell population. Research findings and our inhouse data suggest that endothelial cells are not α-SMA-negative under all circumstances. α-SMA negativity is not an intrinsic property of endothelial cells but can vary depending on extrinsic influences. Lot-specific values for α-SMA in our Certificates of Analysis (CoAs) are therefore not considered meaningful.
Further Information
Ando et al.; Am J Physio l. 1999 May;276(5):H1755-68
Lu et al.; Stem Cells Dev . 2004 Oct;13(5):521-7
Azuma et al.; Biochem Biophys Res Commun . 2009 Mar 13;380(3):620-6
Frid et al.; Circ Res . 2002 Jun 14;90(11):1189-96
Kovacic et al.; J Am Coll Cardiol . 2019 Jan 22;73(2):190-209
Jones et al.; Am J Respir Cell Mol Biol . 1999 Apr;20(4):582-94
Ma et al.; Front Cell Dev Biol . 2020 Apr 21;8:260
Kocher and Madri; In Vitro Cell Dev Biol . 1989 May;25(5):424-34
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
Secretory granules called Weibel-Palade bodies (WPBs) containing Von Willebrand factor (vWF) are more linked to the formation of a confluent endothelial monolayer. Research shows that vWF expression is dynamic and highly dependent on the cell culture conditions such as confluence and passage number. Therefore, having vWF as a quality control marker for each lot of ECs is not really necessary.
Further Information
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
The components of the PromoCell DetachKit may arrive on occasion with a non-uniform color appearance. This phenomenon is known by PromoCell’s Quality Assurance. It is reversible and does not influence the quality of the product.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
Yes, Accutase Solution can be defrosted, aliquoted, and then refrozen. Defrosting: Accutase should be defrosted overnight in the refrigerator or placed in a tub of cold tap water. Do not defrost in a 37°C water bath. Stability: Once thawed, it is stable for at least 2 months in the refrigerator if stored promptly after use.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
This is not advised. Please seed the freshly isolated CD14-monocytes immediately in the Monocyte Attachment Medium. Adding a culturing step will change the biological characteristics of monocytes very rapidly.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
The PBS buffering enhances the Alizarin Red staining (precipitation of the dye) and makes it more intense. Leave the PBS on the cells after staining/washing and analyze the sample immediately, as the dye may bleed upon prolonged storage without embedding.
tools-for-respiratory-research_brochure
PromoCell_Best-Practices-in-Cell-Culture
Standardized culture of assay-ready and fully functional human primary macrophages
The use of antibiotics creates a false sense of security and allows users to develop poor aseptic techniques. This leads to low-level contamination with partially resistant bacteria occurring but being overlooked for a time. This then leads to cells with undetected contamination being cultured for extended periods of time, increasing the risk that contamination will spread throughout the laboratory and eventually antibiotic-resistant strains of bacteria may develop. Mycoplasma infections can also occur more easily, as they are often introduced along with contaminants such as bacteria and fungi. Last but not least, antibiotics are known to have negative effects on the metabolism of eukaryotic cells - more details on this topic can be found in our blog article "Antibiotics in cell culture: friend or enemy".
Further Information