Can you provide a list of cell lines that have been successfully used for tumorsphere formation with PromoCell 3D Tumorsphere Medium XF?

Yes. The following human cancer cell lines have been tested at PromoCell and shown to form tumorspheres when cultured in 3D Tumorsphere Medium XF (C‑28070):

• U‑87 MG
• MCF‑7
• MDA‑MB‑231
• HT‑29
• CT26
• HT1080
• HepG2
• A‑549
• Panc‑1
• LNCaP

In addition, customer feedback indicates successful tumorsphere formation for the following cell lines:

• A‑431 (human epidermal carcinoma)
• 4T1 (mouse mammary carcinoma)
• HCT‑116 (human colorectal carcinoma)
• Capan‑1 (human pancreatic adenocarcinoma)
• PC3 (human prostate cancer)
• C4-2B (human osteotropic prostate cancer)
• NCI‑H23 (human lung adenocarcinoma)
• IMR‑32 (human neuroblastoma)
• A818‑6 (human pancreatic ductal adenocarcinoma)
• HEK293 (human embryonic kidney)
• Calu‑1 (human non‑small‑cell lung cancer)

For experimental details, please refer to the Application Note "Tumorsphere culture of cancer stem cells".