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Cancer Media

Cancer cell culture media allow you to cultivate tumor cells in vitro under defined and reproducible conditions. Our Cancer Media Toolbox is a portfolio of defined, serum-free cell culture media designed for mono-culture and co-culture cell models. It supports applications including 2D and 3D cancer research, immune interaction studies, and patient-derived tumor models. This allows you to develop physiologically relevant systems that preserve tumor heterogeneity and tumor microenvironment interactions, including those involving cancer-associated fibroblasts and cancer stem cells.

Choose the right cancer cell culture medium for your model

The table below provides an overview of our cancer media portfolio, highlighting key formulation characteristics, their suitability for different culture systems and potential applications. Find the best fit for your workflow.

Medium Formulation specification Suitable culture type Potential applications
3D Tumorsphere Medium XF Serum-free and xeno-free, chemically defined; also available as phenol red-free
  • 3D suspension culture (turmospheres/mammospheres)
  • Supports transition from 2D to 3D modes
  • Tumor heterogeneity and metastasis studies
  • Drug screening in physiologically relevant 3D model systems
Primary Cancer Culture System Defined, animal component-free (D-ACF)
  • Primary tumor cell 2D cultures
  • Selective culture of cancer stem cells (CSCs) from biopsies or PDX mouse
  • Tumor heterogeneity studies
  • Patient-derived model generation
Cancer Cell Line Medium XF Serum-free and xeno-free
  • 2D adherent culture
  • Co-culture systems (cancer cells with stromal or immune cells)
  • Drug screening
  • Extracellular vesicles (EV) production studies

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