Is it possible to carry out macrophage differentiation in 384‑well plates? If not, can the cells be detached and re‑plated afterward?
a) Macrophage differentiation in 384‑well plates
At PromoCell, macrophage differentiation is routinely performed in T75 flasks or 6‑well plates.
Differentiation in 384-well formats has not been tested internally.
Based on practical considerations, differentiation in very small well formats is likely to be challenging, mainly because:
At PromoCell, macrophage differentiation is routinely performed in T75 flasks or 6‑well plates.
Differentiation in 384-well formats has not been tested internally.
Based on practical considerations, differentiation in very small well formats is likely to be challenging, mainly because:
- It is difficult to thoroughly wash the wells after the attachment phase.
- Incomplete removal of non‑adherent cells can negatively affect differentiation quality.
- Small volumes increase sensitivity to handling variability.
- Detachment of mature macrophages is possible
- However, re‑attachment is associated with significant cell loss, typically in the range of 30–50%
- This loss should be taken into account when planning downstream experiments.
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