Is it possible to carry out macrophage differentiation in 384‑well plates? If not, can the cells be detached and re‑plated afterward?
a) Macrophage differentiation in 384‑well plates
At PromoCell, macrophage differentiation is routinely performed in T75 flasks or 6‑well plates.
Differentiation in 384-well formats has not been tested internally.
Based on practical considerations, differentiation in very small well formats is likely to be challenging, mainly because:
At PromoCell, macrophage differentiation is routinely performed in T75 flasks or 6‑well plates.
Differentiation in 384-well formats has not been tested internally.
Based on practical considerations, differentiation in very small well formats is likely to be challenging, mainly because:
- It is difficult to thoroughly wash the wells after the attachment phase.
- Incomplete removal of non‑adherent cells can negatively affect differentiation quality.
- Small volumes increase sensitivity to handling variability.
- Detachment of mature macrophages is possible
- However, re‑attachment is associated with significant cell loss, typically in the range of 30–50%
- This loss should be taken into account when planning downstream experiments.
Products relevant to this topic
Monocyte Attachment Medium
Cat No
C-28051
Defined, animal-component free, and protein-free medium for efficient adherence selection of monocytes from freshly isolated human mononuclear cells.
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.
Macrophage Base Medium XF
Cat No
C-28057
User-customizable, serum-free and xeno-free medium for generation of macrophages from fresh monocytes. Medium does not include cytokines.
Macrophage Detachment Solution
Cat No
C-41330
Chemically defined, non-enzymatic detachment solution optimized for gentle detachment of strongly adherent macrophages.