If I isolate fresh CD14⁺ monocytes for M1/M2 macrophage generation, can I culture them for a period of time before differentiation?
No. This is not recommended.
Freshly isolated CD14⁺ monocytes should be seeded immediately into Monocyte Attachment Medium after isolation.
Adding an intermediate culture step before the attachment and differentiation phase is strongly discouraged, because monocytes change their biological characteristics very rapidly in vitro.
To ensure controlled and reproducible macrophage differentiation, freshly isolated CD14⁺ monocytes should therefore be used immediately, without prior cultivation.
Freshly isolated CD14⁺ monocytes should be seeded immediately into Monocyte Attachment Medium after isolation.
Adding an intermediate culture step before the attachment and differentiation phase is strongly discouraged, because monocytes change their biological characteristics very rapidly in vitro.
To ensure controlled and reproducible macrophage differentiation, freshly isolated CD14⁺ monocytes should therefore be used immediately, without prior cultivation.
Products relevant to this topic
Monocyte Attachment Medium
Cat No
C-28051
Defined, animal-component free, and protein-free medium for efficient adherence selection of monocytes from freshly isolated human mononuclear cells.
149.00$
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
1,109.00$
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.
1,109.00$
Macrophage Base Medium XF
Cat No
C-28057
User-customizable, serum-free and xeno-free medium for generation of macrophages from fresh monocytes. Medium does not include cytokines.
238.00$