How many passages can PromoCell melanocytes undergo before losing activity?
PromoCell Normal Human Epidermal Melanocytes (NHEM, NHEM M3) are primary cells. For these cells, we guarantee at least 15 population doublings (PDs) under recommended culture conditions.
In practical terms, this corresponds to approximately 6-8 passages (subcultivations), depending on the seeding density and split ratio used.
However, it is important to note that:
In practical terms, this corresponds to approximately 6-8 passages (subcultivations), depending on the seeding density and split ratio used.
However, it is important to note that:
- While proliferation is guaranteed for 15 PDs, we cannot guarantee that the expression or activity of all melanocyte-specific genes remains unchanged over this period.
- Like all primary cells, melanocytes gradually undergo phenotypic changes during in vitro culture.
Products relevant to this topic
Normal Human Epidermal Melanocytes (NHEM)
Cat No
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Primary Human Epidermal Melanocytes isolated in PMA-containing Melanocyte Growth Medium from the epidermis of juvenile foreskin.
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Normal Human Epidermal Melanocytes (NHEM) adult donor, cultured in M3 Medium
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Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.
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Normal Human Epidermal Melanocytes (NHEM) juvenile foreskin, cultured in M3 Medium
Cat No
C-12422
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.
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