How many passages can PromoCell melanocytes undergo before losing activity?

PromoCell Normal Human Epidermal Melanocytes (NHEM, NHEM M3) are primary cells. For these cells, we guarantee at least 15 population doublings (PDs) under recommended culture conditions.
In practical terms, this corresponds to approximately 6-8 passages (subcultivations), depending on the seeding density and split ratio used.

However, it is important to note that:

• While proliferation is guaranteed for 15 PDs, we cannot guarantee that the expression or activity of all melanocyte-specific genes remains unchanged over this period.
• Like all primary cells, melanocytes gradually undergo phenotypic changes during in vitro culture.

For this reason, we strongly recommend performing critical experiments at low passage numbers, where the cells most closely reflect their in vivo phenotype.