How long does it take to detach primary cells with Accutase (C‑41310)?

Most primary cells detach within approximately 5–15 minutes at 37°C when using accutase solution (C‑41310).
 
Additional notes

• Inactivation of accutase is not required
• Once most of the cells have detached, collect the cell suspension, dilute it with fresh medium, and centrifuge it to remove accutase and EDTA before replating the cells

This helps ensure optimal cell recovery and performance in subsequent culture steps.