How long does it take to detach primary cells with Accutase (C‑41310)?
Most primary cells detach within approximately 5–15 minutes at 37°C when using accutase solution (C‑41310).
Additional notes
Additional notes
- Inactivation of accutase is not required
- Once most of the cells have detached, collect the cell suspension, dilute it with fresh medium, and centrifuge it to remove accutase and EDTA before replating the cells
