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What is the shelf life of primary cells cryopreserved in liquid nitrogen?

PromoCell primary cells are cryopreserved using a computer‑controlled freezing process in the gas phase of liquid nitrogen and subsequently stored in the liquid phase of LN₂.
Cryopreservation in liquid nitrogen is a well‑established method for long‑term storage of primary cells and stem cells. When continuously stored in liquid nitrogen, PromoCell cells can be maintained for more than 10 years without loss of viability.
 
Supporting evidence
Long‑term stability of cryopreserved primary cells has been demonstrated in the literature. For example, Kumar et al. showed that adipose‑derived stem cells stored in liquid nitrogen for approximately 12 years retained viability, stem cell characteristics, differentiation capacity, and regenerative potential.

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