What is the reason for turbid serum?
Serum turbidity is most commonly caused by cryoprecipitation of lipid components that occurs during freezing and thawing.
The more frequently serum is subjected to freeze–thaw cycles, the more pronounced the turbidity typically becomes. This effect does not necessarily indicate contamination or loss of functionality, but rather physical precipitation.
How to minimize serum turbidity
To reduce turbidity, we recommend the following handling practices:
The more frequently serum is subjected to freeze–thaw cycles, the more pronounced the turbidity typically becomes. This effect does not necessarily indicate contamination or loss of functionality, but rather physical precipitation.
How to minimize serum turbidity
To reduce turbidity, we recommend the following handling practices:
- Aliquot the serum before freezing
- Store aliquots at −20°C
- Thaw only the required aliquot immediately before use
- Avoid repeated freeze–thaw cycles
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