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What happens if I grow CD34⁺ progenitors in Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021) without Cytokine Mix E (C‑39890)?

If hematopoietic progenitor cells are cultured in Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021) without Cytokine Mix E (C‑39890), the cells will not survive and will die.
Hematopoietic Progenitor Cell Expansion Medium XF must always be supplemented with Cytokine Mix E or, alternatively, with an appropriate, functionally equivalent cytokine cocktail to support cell survival and proliferation.

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