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What differentiation ratio can be achieved with PromoCell subcutaneous Human White Preadipocytes (HWP)?

Most PromoCell subcutaneous Human White Preadipocyte (HWP) lots achieve a differentiation ratio of > 80-90% when adipogenic differentiation is induced at passage 2 (P2), directly after thawing.
For optimal differentiation performance, PromoCell recommends:
  • using cells that have undergone no more than 4-5 population doublings, and
  • performing differentiation without exceeding one passage after thawing.
As preadipocytes age in culture, their differentiation capacity gradually declines, resulting in lower differentiation ratios.

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