Tumorsphere cell culture: Method and efficiency
Read about the tumorsphere culture and the tumorsphere formation efficiency, including protocols.
Cancer stem cells (CSCs) can be enriched and cultured as extended serial 3D tumorsphere cultures, thus permitting the establishment of a more physiological 3D microenvironment.
In this poster, you will get
- List of cancer cell types to establish successful 3D tumorsphere culture
- 3D Tumorosphere culture protocol
- Tumorsphere formation efficiency (TFE) Assay; detailed protocol including quantification process
Download the poster
Fill in the form to download the PDF.
Related resources
Isolation of patient-derived primary cancer cells
Isolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the primary cancer culture system and how it compares to other established culture methods.
Application Note
Cancer cell culture: Diversity of cells in the tumor microenvironment (TME)
Tumor growth is sustained by a highly diverse microenvironment, the TME. Its complex cellular interactions need serious consideration during Cancer Research.
Application Note
Cancer Cell Culture
Our cancer cell culture portfolio covers a complete range of applications for culturing human malignant cells. Download our brochure to find out more.
Application Note
Growing cancer organoids
Cancer organoids or tumorspheres? Our infographic will help you find the right model for your research.
Application Note
Tumorsphere Culture of Cancer Stem Cells (CSC) with the PromoCell 3D Tumorsphere Medium XF
Find out how to cultivate cancer stem cells in tumorspheres using our defined and xeno-free 3D Tumorsphere Medium. This enables you to characterize such cells and study their malignancy.
Application Note
Recommended products
Cancer Cell Line Medium XF
Serum-free and xeno-free medium for the standardized in vitro cultivation of established cancer cell lines. Fibronectin or vitronectin coating required.
$167.00
NCCD Reagent
NCCD Reagent is part of our Primary Cancer Culture System and is crucial for the pre-treatment of the culture vessel.
$279.00
Primary Cancer Culture System
A complete defined and animal-component free cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts.
$973.00
3D Tumorsphere Medium XF
Serum-free and xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
$158.00