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To what extent will CD34⁺ progenitor cells proliferate in Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021)?

For optimal expansion, human CD34⁺ progenitor cells are cultured in PromoCell Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021) supplemented with Cytokine Mix E (C‑39890) or an alternative cytokine cocktail suitable for progenitor cell expansion.
Cytokine Mix E contains the recombinant human cytokines TPO, SCF, Flt‑3 ligand, and IL‑3, which support robust proliferation of CD34⁺ cells.
 
Under these conditions:
  • Strong expansion of CD34⁺ progenitor cells is typically maintained for approximately 2 weeks.
  • The expansion factor of CD34⁺ cells is usually in the range of 75‑ to 200‑fold, depending on donor material and culture conditions.

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