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Yes you can use accutase to detach Normal Human Cells. Accutase acts very gently on the cells. Cell membranes and surface epitopes will not be harmed. It is therefore mostly used for applications that require unchanged surface markers e.g. for flow cytometry or for detachment of very sensitive cells.
Yes; Accutase Solution can be defrosted; aliquoted; and then refrozen.
Defrosting: Accutase should be defrosted overnight in the refrigerator or placed in a tub of cold tap water. Do not defrost in a 37°C water bath.
Stability: Once thawed; it is stable for at least 2 months in the refrigerator if stored promptly after use.