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NHEK.f are isolated from tissue samples (foreskin) of single donors; aged between 1-10 years. NHEK.f pooled are prepared from the foreskins of 3 individual donors. The cells of each donor are expanded in separate TC vessels and the cells are pooled after secondary culture; before cryopreservation.
Both NHEK from single donor and NHEK pooled are in P2 after thawing.
Yes; the NHEK-GM2 (C-12001; C-12003; C-12005; C-12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C-20021). Using the protocol with the fixed intervals; they grow slightly faster than in Keratinocyte GM2 and proliferate for > 15 PDs.
Conversely; NHEK-GM3 (C-12011; C-12013; C-12015; C-12016) also grow in the existing Keratinocyte Growth Medium 2 (C-20011). When using the classical subcultivation protocol (density > 70% - 90%); they grow slightly slower compared to Keratinocyte GM3; but also reach > 15 PDs.
The NHEK (primary human keratinocytes) are isolated in our serum-free Keratinocyte Growth Medium 2 (C-20011) the NHEK GM3 in our improved serum-free and BPE-free Keratinocyte Growth Medium 3 (C-20021). Both NHEK and NHEK GM3 are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin.
PromoCell's Normal Human Epidermal Keratinocytes (NHEK) from juvenile donors are isolated from both the epidermis of the outer and mucosal (i.e. inner) layers of the foreskin. Thus; each vial contains a mixture of mucosal and cutaneous keratinocytes.