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Supplement Mix for Melanocyte Growth Medium M2 (C-39420) is free of serum and doesn't contain any PMA. The qualitiative and quantitative composition is proprietary.
Note: Melanocyte Growth Medium M2 and NHEM M2 cells were disontinued at the end of 2024.
For the expansion of hematopoietic progenitors (CD133+ cells; CD34+ cells); PromoCell has developed Hematopoietic Progenitor Cell Expansion Medium XF (C-28021); a serum-free and xeno-free formulation. The medium must be supplemented with either Cytokine Mix E (C-39890) or with the user's own mixture of cytokines. Cytokine Mix E is a ready-to-use mix containing recombinant human TPO; SCF; flt3-ligand; and IL-3.
The strong expansion of the progenitor cells in Expansion Medium XF + Cytokine Mix E persists for at least two weeks; resulting in a 200-300x increase of the cell number with only 20-30% differentiated cells.
Note: If starting with CD133+ cells; the CD133 marker is getting lost during this expansion step. The resulting cells are D34+/CD38-/CD133-.
The medium supplemented with Cytokine Mix E is stable for 2 weeks if stored protected from light at 2-8°C.