Search results for: 'C-3'

For the expansion of hematopoietic progenitors (CD133+ cells; CD34+ cells); PromoCell has developed Hematopoietic Progenitor Cell Expansion Medium XF (C-28021); a serum-free and xeno-free formulation. The medium must be supplemented with either Cytokine Mix E (C-39890) or with the user's own mixture of cytokines. Cytokine Mix E is a ready-to-use mix containing recombinant human TPO; SCF; flt3-ligand; and IL-3. The strong expansion of the progenitor cells in Expansion Medium XF + Cytokine Mix E persists for at least two weeks; resulting in a 200-300x increase of the cell number with only 20-30% differentiated cells. Note: If starting with CD133+ cells; the CD133 marker is getting lost during this expansion step. The resulting cells are D34⁺/CD38^-/CD133^-.

The medium supplemented with Cytokine Mix E is stable for 2 weeks if stored protected from light at 2-8°C.

All cytokines in Cytokine Mix E (human TPO SCF flt-3 ligand and IL-3) are produced in E.coli. They are purified by chromatography are free of endotoxins and are tested for their biological activity.

Our Osteoblast Supplement consists of FCS (10 % [v/v] final concentration) which is specifically tested to support optimal growth of Normal Human Osteoblasts.

The Chondrocyte Growth Medium SupplementMix consists of Fetal Calf Serum (final concentration 10 % v/v) which has specifically been tested for the culture of primary chondrocytes. FCS contains a variety of different growth factors which are however not analyzed in more detail. There are no further growth factors added to the SupplementMix.

The cells will die. Hematopoietic Progenitor Cell Expansion Medium XF always must be supplemented with Cytokine Mix E or an appropriate cocktail of cytokines.

Yes it is possible to aliquot the 5 ml Cytokine Mix E (C-39891) into 5 x 1 ml.

The Cytokine Mix M1 and M2 should not be subjected to further freeze/thaw cycles.