Search results for: 'C-2'

MSC Growth Medium 2 (C-28009) is an optimized medium formulation with reduced serum content to allow for more standardized culture conditions (considerably lower lot to lot variation). You can replace MSC Growth Medium by MSC Growth Medium 2. Coating of culture vessels is not necessary. We recommend to plate the cells (hMSCs from bone marrow; adipose tissue; or umbilical cord) at 4;000 cells/cm².

This method produces large numbers of adherent monocytes in only 1.5 h. If the washing steps are properly performed 80-90% purity can be expected. The attached cells are “untouched” since no binding of magnetic microbeads has occured. This also excludes phagocytosis of the microbeads by the monocytes an event which is unfavorable with regard to cellular health. In addition the adherence method is time-saving and cost-effective.

Yes, our MSC Growth Medium XF (# C-28019) contains phenol red. The concentration is confidential. 

But we also supply a phenol red-free variant: Mesenchymal Stem Cell Growth Medium XF, prf (# C-28018).

Our MSC Growth Medium XF (Ready-to-use) provides a xeno-free culture system for human MSCs. It contains all growth factors and supplements except attachment- and spreading factors. Therefore; culture vessels must be precoated with 10 μg/ml human or bovine fibronectin. Protocol: Fibronectin coating Dilute the Fibronection Solution to 10 μg/ml final concentration in Dulbecco´s PBS w/o Calcium and Magnesium. Overlay the culture surface of your tissue culture vessel with an amount of the diluted Fibronectin Solution sufficient to effectively coat the complete surface. Be sure that the entire surface is covered. Place flasks on a level surface at RT for 60 min. Aspirate the excess Fibronectin Solution and use immediately or let air-dry the open vessel under a laminar flow bench. Unused vessels may be stored at 4°C for up to 2 weeks.

The Monocyte Attachment Medium allows for the efficient adherence selection (within 1 hr) of monocytes from freshly isolated mononuclear cells while maintaining optimal cell health. The Time-consuming and costly immunomagnetic purification of monocytes prior to DC generation is not necessary and can be skipped when using this medium.

• DC Base Medium (C-28053) consists of 250 ml Basal Medium + SupplementMix. It does not include cytokines and must therefore be supplemented according to the user's individual needs.

• DC Generation Medium (C-28050) is ready-to-use and consists of 250 ml Basal Medium + SupplementMix + cytokines.

• DC Base Medium XF (C-28054) consists of 250 ml Basal Medium + SupplementMix. It does not include cytokines and must therefore be supplemented according to the user's individual needs.

• DC Generation Medium XF (C-28052) is ready-to-use and consists of 250 ml Basal Medium + SupplementMix + appropriate cytokines.

Both; Base Medium XF and Generation Medium XF have a xeno-free formulation.

The cells sink down to the bottom of the culture vessel but don't really attach. They retain a roundish morphology and can be rinsed off with culture medium easily.

For the expansion of hematopoietic progenitors (CD133+ cells; CD34+ cells); PromoCell has developed Hematopoietic Progenitor Cell Expansion Medium XF (C-28021); a serum-free and xeno-free formulation. The medium must be supplemented with either Cytokine Mix E (C-39890) or with the user's own mixture of cytokines. Cytokine Mix E is a ready-to-use mix containing recombinant human TPO; SCF; flt3-ligand; and IL-3. The strong expansion of the progenitor cells in Expansion Medium XF + Cytokine Mix E persists for at least two weeks; resulting in a 200-300x increase of the cell number with only 20-30% differentiated cells. Note: If starting with CD133+ cells; the CD133 marker is getting lost during this expansion step. The resulting cells are D34⁺/CD38^-/CD133^-.

PromoCell's Osteoblast Basal Medium is an optimized media formulation developed for human osteoblast culture. The exact composition is proprietary.

PromoCell's Endothelial Cell Growth Medium (C-22010) is a complete medium that can be used for the culture of HUVEC after addition of the SupplementMix. Addition of extra FCS is not necessary. The SupplementMix contains FCS (2% v/v final concentration); recombinant growth factors; hormones; and a bovine brain extract that together have mitogenic effects on endothelial cells.