Application Notes

Items 1-12 of 40 Results

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  • Expansion of primitive Hematopoietic Progenitor Cells

    Expansion of primitive Hematopoietic Progenitor Cells

    Researchers are facing difficulties during in vitro expansion of undifferentiated hematopoietic stem cells (HSC). Our Hematopoietic Progenitor Cell (HPC) Expansion Medium XF provides a highly optimized, and xeno-free culture system for human HSC.
  • Real-Time Analysis of Stem Cell Differentiation

    Real-Time Analysis of Stem Cell Differentiation

    Here we describe the real-time analysis of human stem cell differentiation using cell-based assays. This allows for quantification of cell viability, proliferation and apoptosis.
  • Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells

    Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells

    Detailed analysis of human stem cells offers the potential for the specific treatment of diseases by cell-based therapies. With an automated real-time analysis, we assess phenotypic changes during mesenchymal stem cell (MSC) differentiation.
  • Osteogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Osteogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Differentiate mesenchymal stem cells (MSC) into osteoblasts. Read our application note and find out more. The multipotency of these cells is one essential characteristic of MSC, according to the International Society for Cellular Therapy.
  • Neurogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Neurogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Specific culture conditions allow for transdifferentiation of mesenchymal stem cells (MSC) into cells of non-mesenchymal origin such as neurons. To do so, specific culture conditions and stimuli are needed.
  • Chondrogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Chondrogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Managed in vitro differentiation of mesenchymal stem cells (MSC) into chondrocytes. Multipotent adult stem cells are important for various tissues of the musculoskeletal system.
  • Adipogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Adipogenic Differentiation and Analysis of Mesenchymal Stem Cells (MSC)

    Turning mesenchymal stem cells (MSC) into adipocytes is the aim of this application note. Research using MSC is a field of high interest and could ultimately lead to new therapies for treating a variety of lifestyle diseases.
  • Osteoblast Differentiation and Mineralization

    Osteoblast Differentiation and Mineralization

    Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. Their differentiation in vitro and in vivo can be characterized in three stages. We describe analysis of different molecular markers of those processes.
  • Xeno-free MSC culture: high yield, better viability and morphology

    Xeno-free MSC culture: high yield, better viability and morphology

    Are you trying to mimic in vivo conditions in your MSC cultures as closely as possible, but need to avoid serum? Then find out how the combination of PromoCell DXF medium and a unique coating called myMATRIX help you make your conditions highly physiological while maintaining reproducibility.
  • MSC cultivation in single-use stirred tank bioreactors

    MSC cultivation in single-use stirred tank bioreactors

    MSCs have gained significant attention because of their therapeutic potential for many difficult-to-treat diseases. In this app note, we provide a protocol for (GMP) compliant large-scale production of MSCs in a single-use bioreactor using our MSC Growth Medium XF.
  • Cancer organoid model

    Cancer organoid model

    This application note describes a method for easy 3D organoid culture of Primary Human Dermal Fibroblasts (NHDF) and MCF-7 cells in our Cancer Cell Line Medium XF.
  • Generation of Human Airway Organoids from Primary Cells

    Generation of Human Airway Organoids from Primary Cells

    This Application Note describes the generation of functional 3D airway organoids with apical-out or inward-oriented cilia formation based on Primary Human Bronchial Epithelial Cells (HBEpC) as an in vitro model for high-throughput drug screening or large-scale virus-host interaction studies.
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