Human Skeletal Muscle Cells (SkMC)
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Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request).
New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.
SkMC are optimal for in vitro muscle studies. They proliferate very well in our mitogen-rich Skeletal Muscle Cell Growth Medium. Fusion to myotubes with typical multinucleated syncytia can be induced by using our Skeletal Muscle Cell Differentiation Medium.
- Request our GMP grade cell culture media for skeletal muscle cells.
- Our SkMC are now also available from HLA-typed donors.
| Recommended plating density | 3,500 - 7,000 cells per cm2 |
| Passage after thawing | P2 |
| Tested markers | Differentiation capacity to multinucleate syncytia tested |
| Guaranteed population doubling | > 15 |
| Recommended culture media* | C-23060, C-23061 |
*The catalog numbers in this table are for media in ready-to-use packaging.
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