Human Pulmonary Microvascular Endothelial Cells (HPMEC)
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Primary Human Pulmonary Microvascular Endothelial Cells (HPMEC) are isolated from the lung from a single donor. Since lung tissue contains blood and lymphatic capillaries, HPMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle α-actin.
The lung has a vast endothelial surface area, which is essential for the exchange of gases. HPMEC are most appropriate for studying human lung diseases.
- Request our GMP grade cell culture media for endothelial cells.
- Our HPMEC are now also available from HLA-typed donors.
| Recommended plating density | 10,000 - 20,000 cells per cm2 |
| Passage after thawing | P2 |
| Tested markers | CD31 positive, Dil-Ac-LDL uptake positive |
| Guaranteed population doubling | > 15 |
| Recommended culture media* | C-22020, C-22022 |
*The catalog numbers in this table are for media in ready-to-use packaging.
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