Our cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.
Cryopreserved human macrophages are produced from monocytes in our well-proven M1 Macrophage Generation Media XF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using our proprietary, protein-free, and animal-component-free freezing medium, Cryo-SFM or Cryo-SFM Plus. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.
Our M1 Macrophages are now also available from HLA-typed donors.
Figure 1.Human macrophage cell culture in phase contrast