Citations
PromoCell uses the Bioz AI engine to display product citations. This content is currently blocked due to your cookie preferences.
Human Cardiac Myocytes (HCM)
Our Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Our HCM behave like progenitor cells and are not fully differentiated. They do not spontaneously beat in vitro, but unlike freshly isolated rod-shaped myocytes, our HCM can be cultured for at least 15 population doublings. Therefore, when cultured in our Myocyte Growth Medium, HCM are suitable for long-term experiments like investigating the long-term effects of cytokines, toxicological screenings, or cell-cell interactions.
- Request our GMP grade cell culture media for cardiac myocytes.
- Our HCM are now also available from HLA-typed and Diabetes Type I and Type II/Cardiomypathy donors.
Recommended plating density | 10000 - 15000 cells per cm2 |
Passage after thawing | P2 |
Tested markers | Sarcomeric α-actinin positive, slow muscle myosin positive |
Guaranteed population doubling | > 15 |

Short protocol:
- Trypsinize the cells as usual
- Centrifuge and resuspend in suitable cold freezing medium at a density of 1-4 x 106 cells/ml
- Cool down the cells slowly to -80°C (approx. -1°C per min). We recommend to use "Mr. Frosty" from Nalge or "CoolCell" from Biocision; which both provide gradual and controlled cooling rates when placed in a -80°C freezer overnight.
- Transfer the vials into liquid nitrogen for long-term storage
- Mesenchymal Stem Cells (C-12974/C-12971/C-12977) need Fibronectin-coating when grown in PromoCell MSC Growth Medium XF (C-28019) and when differentiated in MSC Neurogenic (C-28015); Adipogenic (C-28016); or Osteogenic (C-28013) Differentiation Media.
- Human monocyte-derived macrophages (C-12914/C-12916/C-12915/C-12917) must be seeded into Fibronectin-coated culture vessels in combination with PromoCell's M1- and M2-Generation Media XF (C-28055; C-28056).
- For efficient induction of osteoblast mineralization with PromoCell's Osteoblast Mineralization Medium (C-27020); the TC plates should be pre-coated with collagen type I.
- Liquid phase storage provides a consistent temperature of -196°C; a longer holding time and a greater vial capacity but involves the risk of contamination issues.
- Storage in the gas phase is very safe with respect to contaminations but the holding time of the cells is shorter and the vial capacity is reduced.








PromoCell uses the Bioz AI engine to display product citations. This content is currently blocked due to your cookie preferences.
Recommended products

Myocyte Growth Medium
Low-serum cell culture medium for myocytes from ventricles of the adult heart.