Human Cardiac Microvascular Endothelial Cells (HCMEC)
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Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle α-actin.
HCMEC play an important role in the physiological regulation of coronary blood flow and capillary exchange. Medical conditions such as hypertrophy, ischemia, hypertension, and diabetes mellitus are associated with endothelial dysfunction.
- Request our GMP grade cell culture media for endothelial cells.
- Our HCMEC are now also available from HLA-typed donors.
| Recommended plating density | 10,000 - 20,000 cells per cm2 |
| Passage after thawing | P3 |
| Tested markers | CD31 positive, Dil-Ac-LDL uptake positive |
| Guaranteed population doubling | > 10 |
| Recommended culture media* | C-22020, C-22022 |
*The catalog numbers in this table are for media in ready-to-use packaging.
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