How often should I change the growth medium when culturing PromoCell Normal Human Cells?
In general, the growth medium should be changed every 2-3 days.
Important notes after thawing
Important notes after thawing
- After thawing, the first medium change should normally be performed after 16–24 hours to remove residual freezing medium.
- If a centrifugation step is performed after thawing and the cells are then seeded, the first medium change should be performed no earlier than 24 hours after seeding. This allows the cells sufficient time to recover and attach after the additional handling step.
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