How many passages can I perform with PromoCell Normal Human Cells?
PromoCell does not determine the number of passages but instead we calculate the population doublings (PD) that can be performed with the cells. The term passage only describes the process of detachment and replating and does not take into account different split ratios. The optimal split ratio is calculated from the actual cell yield after trypsinisation and the recommended plating density. In most of our cell types, the split ratio is usually between 1:3 and 1:6. Using 1:4 splits (i.e. increasing the growth surface by factor 4 each time), 15 doublings are achieved after 6-8 passages. For recommended plating densities, please view the respective Manual, section "Specifications".
Products relevant to this topic
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
Cat No
C-12001
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
693.00$
Normal Human Epidermal Keratinocytes (NHEK) adult, single donor
Cat No
C-12003
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
771.00$
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
Cat No
C-12005
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
492.00$
Normal Human Epidermal Keratinocytes (NHEK) adult, pooled
Cat No
C-12006
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
608.00$
Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskin isolated in Growth Medium 3, single donor
Cat No
C-12011
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
693.00$
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, single donor
Cat No
C-12013
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
758.00$
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
Cat No
C-12015
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
492.00$
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled
Cat No
C-12016
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
608.00$