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Do I need to supplement Endothelial Cell Growth Medium (C‑22010) with additional factors such as FBS when culturing HUVEC?

No. Additional supplementation with FBS or other factors is not necessary.
PromoCell’s Endothelial Cell Growth Medium (C‑22010) is a complete medium for the culture of HUVEC once the SupplementMix has been added.
The SupplementMix already contains:
  • FBS (final concentration 2% v/v)
  • Recombinant growth factors
  • Hormones
  • Bovine brain extract (ECGS) and heparin.
Together, these components provide the mitogenic activity required for endothelial cell growth. Adding extra FBS or supplements is therefore not recommended.

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