5 Items
- Wash the cells with sterile PBS or HepesBSS
- Add undiluted accutase to the culture vessel (2 ml per 25 cm2)
- Incubate at room temperature for 5-15 min or at 37°C for faster detachment
- When the majority of the cells has detached; centrifuge the suspension and resuspend the pellet in fresh medium. In most cases no additional washes or neutralization steps are required.